Quantitative measurement of events in the mammalian unfolded protein response

被引：35

作者：

Shang, J ^{[1
]}

机构：

[1] Univ Texas, SW Med Ctr, Dept Pharmacol, Dallas, TX 75390 USA

来源：

METHODS | 2005年 / 35卷 / 04期

关键词：

D O I：

10.1016/j.ymeth.2004.10.012

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER) triggers the unfolded protein response. IRE1, PERK, ATF6, BiP, EDEM, lipid-linked oligosaccharides (LLOs), and XBP1 directly or indirectly participate in this process. This article provides methods used in our laboratory to quantitatively measure the accumulation of mRNAs encoding BiP and EDEM; splicing of XBP-1; cleavage of ATF6; inhibition of protein synthesis by PERK; and extension of LLOs under control and stress conditions. (c) 2004 Elsevier Inc. All rights reserved.

引用

页码：390 / 394

页数：5

共 22 条

[1] Regulation of the dolichol pathway in human fibroblasts by the endoplasmic reticulum unfolded protein response [J].

Doerrler, WT ;

Lehrman, MA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (23) :13050-13055

[2] Protein translation and folding are coupled by an endoplasmic-reticulum-resident kinase [J].

Harding, HP ;

Zhang, YH ;

Ron, D .

NATURE, 1999, 397 (6716) :271-274

[3] Perk is essential for translational regulation and cell survival during the unfolded protein response [J].

Harding, HP ;

Zhang, YH ;

Bertolotti, A ;

Zeng, HQ ;

Ron, D .

MOLECULAR CELL, 2000, 5 (05) :897-904

[4] Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic reticulum stress [J].

Haze, K ;

Yoshida, H ;

Yanagi, H ;

Yura, T ;

Mori, K .

MOLECULAR BIOLOGY OF THE CELL, 1999, 10 (11) :3787-3799

[5] A novel ER α‐mannosidase‐like protein accelerates ER‐associated degradation [J].

Nobuko Hosokawa ;

Ikuo Wada ;

Kiyotaka Hasegawa ;

Tetuya Yorihuzi ;

Linda O Tremblay ;

Annette Herscovics ;

Kazuhiro Nagata .

The EMBO Reports, 2001, 2 (5) :415-422

[6]

LEE A S, 1992, Current Opinion in Cell Biology, V4, P267, DOI 10.1016/0955-0674(92)90042-B

[7] Oligosaccharide-based information in endoplasmic reticulum quality control and other biological systems [J].

Lehrman, MA .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (12) :8623-8626

[8] Ligand-independent dimerization activates the stress response kinases IRE1 and PERK in the lumen of the endoplasmic reticulum [J].

Liu, CY ;

Schröder, M ;

Kaufman, RJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (32) :24881-24885

[9] Role of EDEM in the release of misfolded glycoproteins from the calnexin cycle [J].

Molinari, M ;

Calanca, V ;

Galli, C ;

Lucca, P ;

Paganetti, P .

SCIENCE, 2003, 299 (5611) :1397-1400

[10]

MORI K, 1993, CELL, V74, P743

← 1 2 3 →