SNAP19 mediates the assembly of a functional core promoter complex (SNAPc) shared by RNA polymerases II and III

被引：71

作者：

Henry, RW

Mittal, V

Ma, BC

Kobayashi, R

Hernandez, N

机构：

[1] Cold Spring Harbor Lab, Cold Spring Harbor, NY 11724 USA

[2] Howard Hughes Med Inst, Cold Spring Harbor, NY 11724 USA

来源：

GENES & DEVELOPMENT | 1998年 / 12卷 / 17期

关键词：

SNAP(c); human snRNA promoters; RNA polymerases; transcription;

D O I：

10.1101/gad.12.17.2664

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The basal transcription factor SNAP(c) binds to the PSE, a coke element in the RNA polymerase II and III human snRNA promoters. SNAP(c) contains at least four subunits, but it has not been possible to assemble a fully defined recombinant SNAP(c). Here we reconstitute SNAP(c) from five recombinant subunits, SNAP43, SNAP45, SNAP50, SNAP190, and a newly identified subunit, SNAP19. This recombinant complex binds specifically to the PSE and directs both RNA polymerase II and III snRNA gene transcription. Thus, the same core SNAP(c) nucleates the assembly of two classes of initiation complexes.

引用

页码：2664 / 2672

页数：9

共 23 条

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