Sulfated di-, tri- and tetraantennary N-glycans in human Tamm-Horsfall glycoprotein

被引：63

作者：

Van Rooijen, JJM ^{[1
]}

Kamerling, JP ^{[1
]}

Vliegenthart, JFG ^{[1
]}

机构：

[1] Univ Utrecht, Bijvoet Ctr, Dept Bioorgan Chem, NL-3508 TB Utrecht, Netherlands

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1998年 / 256卷 / 02期

关键词：

Tamm-Horsfall glycoprotein; carbohydrate; NMR; sulfated N-glycan;

D O I：

10.1046/j.1432-1327.1998.2560471.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The primary structures of 32 sulfated di-, tri- and tetraantennary N-glycans of human Tamm-Horsfall glycoprotein (THP) have been determined. THP was isolated from the urine of one healthy male donor. The intact carbohydrate chains were released by PNGase-F and fractionated via FPLC on Resource Q, HPLC on LiChrosorb-NH2, and high-pH anion-exchange chromatography on CarboPac PA-1. Characterizations were performed using 500-MHz and 600-MHz H-1-NMR spectroscopy, in combination with sialidase treatments. The type of characterized N-glycans ranged from monosulfated to trisulfated N-glycans, whereby the sulfate groups were present as 3-O-sulfated Gal (Gal3S) and 4-O-sulfated GalNAc (GalNAc4S). A compilation of the established structures is shown below. [GRAPHICS]

引用

页码：471 / 487

页数：17

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