Synthesis and characterization of small circular double-stranded RNAs

被引：15

作者：

Abe, Naoko ^{[1
]}

Abe, Hiroshi ^{[1
]}

Ohshiro, Takahito ^{[2
]}

Nakashima, Yuko ^{[1
]}

Maeda, Mizuo ^{[2
]}

Ito, Yoshihiro ^{[1
]}

机构：

[1] RIKEN, Nano Med Engn Lab, Adv Sci Inst, Wako, Saitama 3510198, Japan

[2] RIKEN, Bioengn Lab, Adv Sci Inst, Wako, Saitama 3510198, Japan

来源：

CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 07期

关键词：

SCANNING FORCE MICROSCOPY; MAMMALIAN-CELLS; DNA; INTERFERENCE; FLEXIBILITY; HAMMERHEAD; LIGATION; SEQUENCE; DUPLEXES; POTENT;

D O I：

10.1039/c0cc04551a

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Small circular double-stranded RNAs (dsRNAs) were synthesized. The structural analysis by atomic force microscopy gave direct images for the interpretation of the structural strain present in circular dsRNAs. Finally, we demonstrated that circular dsRNA caused RNAi effect in cells.

引用

页码：2125 / 2127

页数：3

共 32 条

[1] Dumbbell-shaped nanocircular RNAs for RNA interference [J].

Abe, Naoko ;

Abe, Hiroshi ;

Ito, Yoshihiro .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (49) :15108-+

[2] Mechanisms of alternative pre-messenger RNA splicing [J].

Black, DL .

ANNUAL REVIEW OF BIOCHEMISTRY, 2003, 72 :291-336

[3] Improved silencing properties using small internally segmented interfering RNAs [J].

Bramsen, Jesper B. ;

Laursen, Maria B. ;

Damgaard, Christian K. ;

Lena, Suzy W. ;

Babu, B. Ravindra ;

Wengel, Jesper ;

Kjems, Jorgen .

NUCLEIC ACIDS RESEARCH, 2007, 35 (17) :5886-5897

[4] CIRCULAR DNA-MOLECULES IMAGED IN AIR BY SCANNING FORCE MICROSCOPY [J].

BUSTAMANTE, C ;

VESENKA, J ;

TANG, CL ;

REES, W ;

GUTHOLD, M ;

KELLER, R .

BIOCHEMISTRY, 1992, 31 (01) :22-26

[5] Potent RNAi by short RNA triggers [J].

Chu, Chia-Ying ;

Rana, Tariq M. .

RNA, 2008, 14 (09) :1714-1719

[6] Single-strand-specific nucleases [J].

Desai, NA ;

Shankar, V .

FEMS MICROBIOLOGY REVIEWS, 2003, 26 (05) :457-491

[7] ATOMIC-RESOLUTION STRUCTURE OF THE CELLULOSE SYNTHASE REGULATOR CYCLIC DIGUANYLIC ACID [J].

EGLI, M ;

GESSNER, RV ;

WILLIAMS, LD ;

QUIGLEY, GJ ;

VANDERMAREL, GA ;

VANBOOM, JH ;

RICH, A ;

FREDERICK, CA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (08) :3235-3239

[8] Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [J].

Elbashir, SM ;

Harborth, J ;

Lendeckel, W ;

Yalcin, A ;

Weber, K ;

Tuschl, T .

NATURE, 2001, 411 (6836) :494-498

[9] Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [J].

Fire, A ;

Xu, SQ ;

Montgomery, MK ;

Kostas, SA ;

Driver, SE ;

Mello, CC .

NATURE, 1998, 391 (6669) :806-811

[10]

Frieden M, 1999, ANGEW CHEM INT EDIT, V38, P3654

← 1 2 3 4 →