Functionalization of nitrocellulose membranes using ammonia plasma for the covalent attachment of antibodies for use in membrane-based immunoassays

被引：34

作者：

Ben Rejeb, S

Tatoulian, M

Khonsari, FA

Durand, NF

Martel, A

Lawrence, JF

Amouroux, J

Le Goffic, F

机构：

[1] Ecole Natl Super Chim Paris, Lab Biotechnol Environm, UMR 7576, CNRS, F-75231 Paris 05, France

[2] Hlth Canada, Hlth Protect Branch, Bur Chem Safety, Food Directorate,Food Res Div, Ottawa, ON K1A 0L2, Canada

[3] Ecole Natl Super Chim Paris, Lab Genie Procedes Plasma, F-75231 Paris 05, France

来源：

ANALYTICA CHIMICA ACTA | 1998年 / 376卷 / 01期

关键词：

immobilization; enzyme immunoassay; affinity membranes; environmental screening test; herbicide; isoproturon; plasma process; polymer functionalization;

D O I：

10.1016/S0003-2670(98)00452-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A plasma process using NH3 was developed in order to incorporate amine functions on the surface of nitrocellulose membranes for an oriented covalent attachment of antibodies through their oxidized carbohydrate moieties. An average covalent binding capacity of 60 mu g/cm(2) of antibodies was reached. The potential use of this process in developing environmental immunochemical techniques was demonstrated. (C) 1998 Published by Elsevier Science B.V. All rights reserved.

引用

页码：133 / 138

页数：6

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