Immunohistochemical evidence for the presence of tryptophan hydroxylase in the brains of insects as revealed by sheep anti-tryptophan hydroxylase polyclonal antibody

被引：4

作者：

Bao, Xuexiang ^{[1
]}

Tian, Ximei ^{[2
]}

Zhao, Zhifu ^{[1
]}

Qu, Yutang ^{[1
]}

Wang, Bin ^{[1
]}

Zhang, Jinbei ^{[1
]}

Liu, Tianyi ^{[1
]}

Yang, Lina ^{[1
]}

Lv, Jiye ^{[3
]}

Song, Chuantao ^{[4
]}

机构：

[1] NE Normal Univ, Sch Life Sci, Lab Insect Brain Neurobiol, Changchun 130024, Peoples R China

[2] Jilin Univ, Minist Educ, Key Lab Terrain Machine Bion Engn, Changchun 130022, Peoples R China

[3] Jilin Prov Sericultural Sci Res Inst, Jilin 132200, Peoples R China

[4] NE Normal Univ, Sch Urban & Environm Sci, Changchun 130024, Peoples R China

来源：

CELL AND TISSUE RESEARCH | 2008年 / 332卷 / 03期

基金：

中国国家自然科学基金;

关键词：

tryptophan hydroxylase; serotonin; immunohistochemistry; aromatic amino acid hydroxylase; brain; insects;

D O I：

10.1007/s00441-008-0596-6

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Immediately following the discovery of tryptophan hydroxylase in Drosophila, we demonstrated the presence of tryptophan hydroxylase in the brain of the beetle Harmonia axyridis (Coleoptera: Coccinellidae). However, whether tryptophan hydroxylase is present in the brains of other insects is still a matter of discussion. In the current study, sheep anti-tryptophan hydroxylase polyclonal antibody has been applied to test for tryptophan hydroxylase immunoreactivity in a broader taxonomic range of insect brains, including holometabolous and hemimetabolous insects: one species each of Coleoptera, Hymenoptera, Diptera, and Blattaria, and two species of Lepidoptera. All species show consistent tryptophan hydroxylase immunoreactivity with distribution patterns matching that of serotonin. The immuno-positive results of such an antibody in brains from diverse orders of insects suggest that specific tryptophan hydroxylase responsible for central serotonin synthesis is probably present in the brains of all insects.

引用

页码：555 / 563

页数：9

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