Neuronal patterning by BMPs: a requirement for GDF7 in the generation of a discrete class of commissural interneurons in the mouse spinal cord

被引:274
作者
Lee, KJ [1 ]
Mendelsohn, M [1 ]
Jessell, TM [1 ]
机构
[1] Columbia Univ, Howard Hughes Med Inst, Dept Biochem & Mol Biophys, Ctr Neurobiol, New York, NY 10032 USA
关键词
BMPs; neural patterning spinal cord; commissural neurons;
D O I
10.1101/gad.12.21.3394
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Inductive factors are known to direct the regional differentiation of the vertebrate central nervous system (CNS) but their role in the specification of individual neuronal cell types is less clear. We have examined the function of GDF7, a BMP family member expressed selectively by roof plate cells, in the generation of neuronal cell types in the dorsal spinal cord. We find that GDF7 can promote the differentiation in vitro of two dorsal sensory interneuron classes, D1A and D1B neurons. In Gdf7-null mutant embryos, the generation of D1A neurons is eliminated but D1B neurons and other identified dorsal interneurons are unaffected. These findings show that GDF7 is an inductive signal from the roof plate required for the specification of neuronal identity in the dorsal spinal cord and that GDF7 and other BMP family members expressed by the roof plate have non-redundant functions in vivo. More generally, these results suggest that BMP signaling may have a prominent role in the assignment of neuronal identity within the mammalian CNS.
引用
收藏
页码:3394 / 3407
页数:14
相关论文
共 62 条
  • [1] A MAMMALIAN HELIX-LOOP-HELIX FACTOR STRUCTURALLY RELATED TO THE PRODUCT OF DROSOPHILA PRONEURAL GENE ATONAL IS A POSITIVE TRANSCRIPTIONAL REGULATOR EXPRESSED IN THE DEVELOPING NERVOUS-SYSTEM
    AKAZAWA, C
    ISHIBASHI, M
    SHIMIZU, C
    NAKANISHI, S
    KAGEYAMA, R
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (15) : 8730 - 8738
  • [2] ALTMAN J, 1984, ADV ANAT EMBRYOL CEL, V85, P1
  • [3] POTENT ECTOPIC BONE-INDUCING ACTIVITY OF BONE MORPHOGENETIC PROTEIN-4/7 HETERODIMER
    AONO, A
    HAZAMA, M
    NOTOYA, K
    TAKETOMI, S
    YAMASAKI, H
    TSUKUDA, R
    SASAKI, S
    FUJISAWA, Y
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 210 (03) : 670 - 677
  • [4] Arkell R, 1997, DEVELOPMENT, V124, P1
  • [5] CONTROL OF CELL PATTERN IN THE NEURAL-TUBE - REGULATION OF CELL-DIFFERENTIATION BY DORSALIN-1, A NOVEL TGF-BETA FAMILY MEMBER
    BASLER, K
    EDLUND, T
    JESSELL, TM
    YAMADA, T
    [J]. CELL, 1993, 73 (04) : 687 - 702
  • [6] Evolutionary conservation of sequence and expression of the bHLH protein Atonal suggests a conserved role in neurogenesis
    BenArie, N
    McCall, AE
    Berkman, S
    Eichele, G
    Bellen, HJ
    Zoghbi, HY
    [J]. HUMAN MOLECULAR GENETICS, 1996, 5 (09) : 1207 - 1216
  • [7] Limb and kidney defects in Lmx1b mutant mice suggest an involvement of LMX1B in human nail patella syndrome
    Chen, H
    Lun, Y
    Ovchinnikov, D
    Kokubo, H
    Oberg, KC
    Pepicelli, CV
    Gan, L
    Lee, B
    Johnson, RL
    [J]. NATURE GENETICS, 1998, 19 (01) : 51 - 55
  • [8] Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene function
    Chiang, C
    Ying, LTT
    Lee, E
    Young, KE
    Corden, JL
    Westphal, H
    Beachy, PA
    [J]. NATURE, 1996, 383 (6599) : 407 - 413
  • [9] Midbrain development induced by FGF8 in the chick embryo
    Crossley, PH
    Martinez, S
    Martin, GR
    [J]. NATURE, 1996, 380 (6569) : 66 - 68
  • [10] DICKINSON ME, 1995, DEVELOPMENT, V121, P2099