POTENT ECTOPIC BONE-INDUCING ACTIVITY OF BONE MORPHOGENETIC PROTEIN-4/7 HETERODIMER

被引：220

作者：

AONO, A ^{[1
]}

HAZAMA, M ^{[1
]}

NOTOYA, K ^{[1
]}

TAKETOMI, S ^{[1
]}

YAMASAKI, H ^{[1
]}

TSUKUDA, R ^{[1
]}

SASAKI, S ^{[1
]}

FUJISAWA, Y ^{[1
]}

机构：

[1] TAKEDA CHEM IND LTD,DRUG SAFETY RES LABS,OSAKA 532,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1995年 / 210卷 / 03期

关键词：

D O I：

10.1006/bbrc.1995.1712

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have purified and characterized recombinant Xenopus bone morphogenetic proteins (xBMPs): homodimers of xBMP-4, 7 and heterodimers (xBMP-4/7) produced by a baculovirus expression system. Highly purified xBMPs had homogeneous NH2-termini predicted from a consensus motif, Arg-X-X-Arg, while they possessed diverse sugar chains. Implantation of xBMPs together with pure collagen carrier in rats induced new bone formation in a dose-dependent manner. The xBMP-4/7 heterodimer showed the strongest activity, with an effective dose of 1-30 mu g, while more than 10 mu g of xBMP-4 or 7 homodimer was required for a significant effect. Histological examination revealed that xBMP-4/7 implants showed intramembranous ossification without chondrogenesis. In primary cultures of rat bone marrow stromal cells, xBMP-4/7 induced alkaline phosphatase 3-fold more strongly than xBMP-7 and 20-fold more than xBMP-4. These results suggest that the heterodimeric form of BMP would generate the strongest signal triggering osteogenic differentiation of osteoprogenitor cells in adult tissues. (C) 1995 Academic Press, Inc.

引用

页码：670 / 677

页数：8

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