Cellobiose dehydrogenase is essential for wood invasion and nonessential for kraft pulp delignification by Trametes versicolor

被引:54
作者
Dumonceaux, T
Bartholomew, K
Valeanu, L
Charles, T
Archibald, F
机构
[1] Pulp & Paper Res Inst Canada, Pointe Claire, PQ H9R 3J9, Canada
[2] Cent Connecticut State Univ, Dept Biol Sci, New Britain, CT 06050 USA
[3] McGill Univ, Dept Nat Resources Sci, Ste Anne De Bellevue, PQ H9X 3V9, Canada
[4] Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada
关键词
D O I
10.1016/S0141-0229(01)00407-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Cellobiose dehydrogenase (CDH)-deficient strains of the basidiomycete Trametes versicolor were produced by transforming protoplasts of strain 52J with a plasmid carrying the T. versicolor cdh gene disrupted with a phleomycin resistance cassette. Of 143 phleomycin-resistant colonies analyzed, 3 did not produce measurable CDH during two successive two-week culture periods. Two of these mutants were shown to, lack functional CDH when grown in CDH induction medium. They biobleached and delignified industrial unbleached kraft pulp as efficiently as did wild-type T. versicolor, indicating that CDH is not required for the degradation and biobleaching of kraft lignin. The ability to degrade C-14-guaiacyl dehydrogenative polymerizate (synthetic lignin) also appeared to be unaffected. However, compared to the parent strain, all three mutants grew poorly on minimal agar with highly crystalline cellulose as the sole carbon source. This difference was not observed on non-crystalline, carbohydrates. All three mutants had a greatly decreased ability to colonize and degrade both seasoned and fresh native white birch wood, a natural substrate of T. versicolor. The dramatic decrease in T. versicolor 52J's ability to invade and grow on birch wood caused by the loss of its secreted CDH strongly suggests that this enzyme is essential to its wood invading and degrading niche in the forest ecosystem. (C) 2001 Elsevier Science Inc. All rights reserved.
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页码:478 / 489
页数:12
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