Decay-accelerating factor (DAF/CD55) is a functional active element of the LPS receptor complex

被引：20

作者：

Heine, H ^{[1
]}

Ulmer, AJ ^{[1
]}

El-Samalouti, VT ^{[1
]}

Lentschat, A ^{[1
]}

Hamann, L ^{[1
]}

机构：

[1] Res Ctr Borstel, Ctr Med & Biosci, D-23845 Borstel, Germany

来源：

JOURNAL OF ENDOTOXIN RESEARCH | 2001年 / 7卷 / 03期

关键词：

D O I：

10.1177/09680519010070030601

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Previously, we identified an 80 kDa membrane protein (LMP80) that is capable of binding to LPS and lipid A in the presence of LBP and sCD14. LMP80 could also be detected after immuno-coprecipitation of cell membranes with LPS and lipid A, indicating a physical contact of LMP80 and LPS/lipid A. Further analysis and peptide sequencing revealed that LMP80 is identical to CD55 (decay accelerating factor, DAF), a regulatory molecule of the complement cascade. Transfection of LPS-hyporesponsive Chinese hamster ovary (CHO) cells with human CD55 resulted in the translocation of NF-kappaB upon stimulation with LPS or lipid A. Our results demonstrate a new functional role of CD55 as a molecule able to mediate LPS-induced activation of cells that may be part of a multimeric LPS receptor complex.

引用

页码：227 / 231

页数：5

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