Sensitive Detection of Bacterial DNA by Magnetic Nanoparticles

被引：30

作者：

Goransson, Jenny ^{[1
,2
]}

De La Torre, Teresa Zardan Gomez ^{[1
]}

Stromberg, Mattias ^{[1
]}

Russell, Camilla ^{[2
]}

Svedlindh, Peter ^{[1
]}

Stromme, Maria ^{[1
]}

Nilsson, Mats ^{[2
]}

机构：

[1] Uppsala Univ, Dept Engn Sci, Angstrom Lab, SE-75121 Uppsala, Sweden

[2] Uppsala Univ, Dept Genet & Pathol, Rudbeck Lab, SE-75185 Uppsala, Sweden

来源：

ANALYTICAL CHEMISTRY | 2010年 / 82卷 / 22期

关键词：

POLYMERASE-CHAIN-REACTION; NANOBEAD DETECTION ASSAY; CIRCLE AMPLIFICATION; OLIGONUCLEOTIDES; IDENTIFICATION; REPLICATION; DIAGNOSTICS; SEQUENCES;

D O I：

10.1021/ac102133e

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This work presents sensitive detection of bacterial genomic DNA using a magnetic nanoparticle-based substrate-free method. For the first time, such a method is employed for detection of a clinically relevant analyte by implementing a solid-phase-based molecular probing and amplification protocol that can be executed in 80 min. The molecular detection and amplification protocol is presented and verified on samples containing purified genomic DNA from Escherichia coli cells, showing that as few as 50 bacteria can be detected. This study moves the use of volume-amplified magnetic nanoparticles one step further toward rapid, sensitive, and selective infectious diagnostics.

引用

页码：9138 / 9140

页数：3

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