Controlled release of Chol-TEG-DNA from nano- and micropatterned SU-8 surfaces by a spreading lipid film

被引：15

作者：

Erkan, Yavuz ^{[1
]}

Halma, Kiona ^{[1
]}

Czolkos, Ilja ^{[1
]}

Jesorka, Aldo ^{[1
]}

Dommersnes, Paul ^{[2
]}

Kumar, Ravindra ^{[3
]}

Brown, Tom ^{[3
]}

Orwar, Owe ^{[1
]}

机构：

[1] Chalmers, Dept Chem & Biol Engn, S-41296 Gothenburg, Sweden

[2] Univ Paris Diderot, MSC, F-75205 Paris, France

[3] Univ Southampton, Sch Chem, Southampton SO17 1BJ, Hants, England

来源：

NANO LETTERS | 2008年 / 8卷 / 01期

关键词：

D O I：

10.1021/nl0725087

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We report the controlled release of immobilized cholesteryl-tetraethyleneglycol-DNA (chol-DNA) from micropatterned SU-8 surfaces by a spreading lipid film. The release of chol-DNA is rapid and on the order of the spreading rate of the lipid film beta = 1-3 mu m(2)/s (similar to 10(5) molecules of DNA per second). The lipid film serves as a poor solvent for the DNA adduct, which upon contact redistributes into the aqueous phase. Thus, the release of DNA is accompanied by a change in surface hydrophobicity. The method can be used for creating arbitrary concentration profiles of DNA in solution over time or to dynamically change surface properties on demand in, for example, micro- and nanofluidic devices. Examples of DNA release from spiral, comb, meander, and triangular as well as from nanoscale SU-8 lanes are shown.

引用

页码：227 / 231

页数：5

共 19 条

[1] High resolution 100 kV electron beam lithography in SU-8 [J].

Bilenberg, B. ;

Jacobsen, S. ;

Schmidt, M. S. ;

Skjolding, L. H. D. ;

Shi, P. ;

Boggild, P. ;

Tegenfeldt, J. O. ;

Kristensen, A. .

MICROELECTRONIC ENGINEERING, 2006, 83 (4-9) :1609-1612

[2] Manipulating the biochemical nanoenvironment around single molecules contained within vesicles [J].

Chiu, DT ;

Wilson, CF ;

Karlsson, A ;

Danielsson, A ;

Lundqvist, A ;

Strömberg, A ;

Ryttsén, F ;

Davidson, M ;

Nordholm, S ;

Orwar, O ;

Zare, RN .

CHEMICAL PHYSICS, 1999, 247 (01) :133-139

[3] Controlled formation and mixing of two-dimensional fluids [J].

Czolkos, Ilja ;

Erkan, Yavuz ;

Dommersnes, Paul ;

Jesorka, Aldo ;

Orwar, Owe .

NANO LETTERS, 2007, 7 (07) :1980-1984

[4] Direct immobilization of cholesteryl-TEG-modified oligonucleotides onto hydrophobic SU-8 surfaces [J].

Erkan, Yavuz ;

Czolkos, Ilja ;

Jesorka, Aldo ;

Wilhelmsson, L. Marcus ;

Orwar, Owe .

LANGMUIR, 2007, 23 (10) :5259-5263

[5] Micro- and nanotechnology via reaction-diffusion [J].

Grzybowski, BA ;

Bishop, KJM ;

Campbell, CJ ;

Fialkowski, M ;

Smoukov, SK .

SOFT MATTER, 2005, 1 (02) :114-128

[6] Light-regulated release of DNA and its delivery to nuclei by means of photolabile gold nanoparticles [J].

Han, Gang ;

You, Chang-Cheng ;

Kim, Byoung-Jin ;

Turingan, Rosemary S. ;

Forbes, Neil S. ;

Martin, Craig T. ;

Rotello, Vincent M. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (19) :3165-3169

[7] Spatially controlled electro-stimulated DNA adsorption and desorption for biochip applications [J].

Hook, AL ;

Thissen, H ;

Hayes, JP ;

Voelcker, NH .

BIOSENSORS & BIOELECTRONICS, 2006, 21 (11) :2137-2145

[8] Surface manilpulation of biomolecules for cell microarray applications [J].

Hook, Andrew L. ;

Thissen, Helmut ;

Voelcker, Nicolas H. .

TRENDS IN BIOTECHNOLOGY, 2006, 24 (10) :471-477

[9] Electroinjection of colloid particles and biopolymers into single unilamellar liposomes and cells for bioanalytical applications [J].

Karlsson, M ;

Nolkrantz, K ;

Davidson, MJ ;

Strömberg, A ;

Ryttsén, F ;

Åkerman, B ;

Orwar, O .

ANALYTICAL CHEMISTRY, 2000, 72 (23) :5857-5862

[10] Modular chemical mechanism predicts spatiotemporal dynamics of initiation in the complex network of hemostasis [J].

Kastrup, Christian J. ;

Runyon, Matthew K. ;

Shen, Feng ;

Ismagilov, Rustern F. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (43) :15747-15752

← 1 2 →