A novel approach to identify proteins modified by nitric oxide: the HIS-TAG switch method

被引：67

作者：

Camerini, Serena

Polci, Maria L.

Restuccia, Umberto

Usuelli, Vera

Malgaroli, Antonio

Bachi, Angela

机构：

[1] Ist Sci San Raffaele, DIBIT, Mass Spectrometry Unit, I-20132 Milan, Italy

[2] Univ Vita Salute San Raffaele, I-20132 Milan, Italy

来源：

JOURNAL OF PROTEOME RESEARCH | 2007年 / 6卷 / 08期

关键词：

S-nitrosylation; proteomics; mass spectrometry; post-translational modifications; nitric oxide;

D O I：

10.1021/pr0701456

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

S-nitrosylation is emerging as an important signaling mechanism that regulates a broad range of cellular functions. The recognition of Cysteine residues that undergo S-nitrosylation is crucial to elucidate how this modification modulates protein activity. We report here a novel strategy, defined His-tag switch, which allows the purification and identification of S-nitrosylated proteins and the unambiguous localization of the modified cysteine residues by mass spectrometry analysis.

引用

页码：3224 / 3231

页数：8

共 52 条

[41] Nitric oxide inhibits an interaction between JNK1 and c-Jun through nitrosylation
Park, Hee-Sae
Mo, Jung-Soon
Choi, Eui-Ju
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 351 (01) : 281 - 286
[42] A REQUIREMENT FOR THE INTERCELLULAR MESSENGER NITRIC-OXIDE IN LONG-TERM POTENTIATION
SCHUMAN, EM
MADISON, DV
[J]. SCIENCE, 1991, 254 (5037) : 1503 - 1506
[43] Mass spectrometric sequencing of proteins from silver stained polyacrylamide gels
Shevchenko, A
Wilm, M
Vorm, O
Mann, M
[J]. ANALYTICAL CHEMISTRY, 1996, 68 (05) : 850 - 858
[44] S-nitrosylation of NSF controls membrane trafficking
Söllner, TH
Sequeira, S
[J]. CELL, 2003, 115 (02) : 127 - 129
[45] (S)NO signals: Translocation, regulation, and a consensus motif
Stamler, JS
Toone, EJ
Lipton, SA
Sucher, NJ
[J]. NEURON, 1997, 18 (05) : 691 - 696
[46] Studying the S-nitrosylation of model peptides and eNOS protein by mass spectrometry
Taldone, FS
Tummala, M
Goldstein, EJ
Ryzhov, V
Ravi, K
Black, SM
[J]. NITRIC OXIDE-BIOLOGY AND CHEMISTRY, 2005, 13 (03): : 176 - 187
[47] S-Nitrosylated protein-disulphide isomerase links protein misfolding to neurodegeneration
Uehara, Takashi
Nakamura, Tomohiro
Yao, Dongdong
Shi, Zhong-Qing
Gu, Zezong
Ma, Yuliang
Masliah, Eliezer
Nomura, Yasuyuki
Lipton, Stuart A.
[J]. NATURE, 2006, 441 (7092) : 513 - 517
[48] Nitric oxide protects against mitochondrial permeabilization induced by glutathione depletion: Role of S-nitrosylation?
Whiteman, M
Chua, YL
Zhang, DW
Duan, W
Liou, YC
Armstrong, JS
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2006, 339 (01) : 255 - 262
[49] Structural and biochemical studies of p21Ras S-nitrosylation and nitric oxide-mediated guanine nucleotide exchange
Williams, JG
Pappu, K
Campbell, SL
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (11) : 6376 - 6381
[50] INHIBITION OF CYTOCHROMES-P450 BY NITRIC-OXIDE AND A NITRIC OXIDE-RELEASING AGENT
WINK, DA
OSAWA, Y
DARBYSHIRE, JF
JONES, CR
ESHENAUR, SC
NIMS, RW
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1993, 300 (01) : 115 - 123

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