Strain-specific differentiation of environmental Escherichia coli isolates via denaturing gradient gel electrophoresis (DGGE) analysis of the 16S-23S intergenic spacer region

被引:32
作者
Buchan, A
Alber, M [1 ]
Hodson, RE
机构
[1] Univ Georgia, Dept Marine Sci, Athens, GA 30602 USA
[2] Univ Georgia, Dept Microbiol, Athens, GA 30602 USA
关键词
16S-23S; ISR; DGGE; strain differentiation; fecal coliform; Escherichia coli;
D O I
10.1111/j.1574-6941.2001.tb00817.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Denaturing gradient gel electrophoresis (DGGE) was applied to the 16S-23S rRNA intergenic spacer region (ISR) as a means to evaluate strain level differences in Escherichia coli. The ISRs of 81 environmental E. coli isolates obtained from bovine, poultry, and human sources yielded a total of 41 unique DGGE banding patterns, with identical patterns and common bands within each source and no overlapping patterns among sources. An additional 51 isolates from two nearby streams yielded 45 unique banding patterns with no overlap between sites. However, two of the isolates from the streams had identical banding patterns to those from two of the source isolates, resulting in a total of 84 unique DGGE banding patterns out of 132 isolates identified in this study. These results revealed high diversity among environmental E. coli isolates, which made it difficult to unambiguously ascribe strains found in water samples to specific host organisms. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Microbiological Societies.
引用
收藏
页码:313 / 321
页数:9
相关论文
共 48 条
[11]   LENGTH-INDEPENDENT SEPARATION OF DNA RESTRICTION FRAGMENTS IN 2-DIMENSIONAL GEL-ELECTROPHORESIS [J].
FISCHER, SG ;
LERMAN, LS .
CELL, 1979, 16 (01) :191-200
[12]   THE LONG-TERM SURVIVAL OF ESCHERICHIA-COLI IN RIVER WATER [J].
FLINT, KP .
JOURNAL OF APPLIED BACTERIOLOGY, 1987, 63 (03) :261-270
[13]  
FRETER R, 1983, CURRENT PERSPECTIVES, P105
[14]   Comparison of the small 16S to 23S intergenic spacer region (ISR) of the rRNA operons of some Escherichia coli strains of the ECOR collection and E-coli K-12 [J].
GarciaMartinez, J ;
MartinezMurcia, A ;
Anton, AI ;
RodriguezValera, F .
JOURNAL OF BACTERIOLOGY, 1996, 178 (21) :6374-6377
[15]  
GELDREICH EE, 1969, J WATER POLLUT CON F, V41, pR336
[16]  
Geldreich EE., 1966, WATER POLLUTION CONT
[17]  
Greensberg A E., 1992, Standard methods for the examination of water and wastewater
[18]  
GURIJALA V, 1980, MICROBIAL ECOL, V20, P231
[19]   New approaches to typing and identification of bacteria using the 16S-23S rDNA spacer region [J].
Gurtler, V ;
Stanisich, VA .
MICROBIOLOGY-SGM, 1996, 142 :3-16
[20]  
Hagedorn C, 1999, APPL ENVIRON MICROB, V65, P5522