Lipopolysaccharide prevents doxorubicin-induced apoptosis in RAW 264.7 macrophage cells by inhibiting p53 activation

被引：31

作者：

Hassan, F ^{[1
]}

Islam, S ^{[1
]}

Mu, MM ^{[1
]}

Ito, H ^{[1
]}

Koide, N ^{[1
]}

Mori, I ^{[1
]}

Yoshida, T ^{[1
]}

Yokochi, T ^{[1
]}

机构：

[1] Aichi Med Univ, Sch Med, Dept Microbiol & Immunol, Aichi 4801195, Japan

来源：

MOLECULAR CANCER RESEARCH | 2005年 / 3卷 / 07期

关键词：

D O I：

10.1158/1541-7786.MCR-05-0046

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The effect of lipopolysaccharide on doxorubicin-induced cell death was studied by using mouse RAW 264.7 macrophage cells. Pretreatment with lipopolysaccharide at 10 ng/mL prevented doxorubicin-induced cell death and the inhibition was roughly dependent on the concentration of lipopolysaccharide. Posttreatment with lipopolysaccharide for 1 hour also prevented doxorubicin-induced cell death. Lipopolysaccharide inhibited DNA fragmentation and caspase-3 activation in doxorubicin-treated RAW 264.7 cells, suggesting the prevention of doxorubicin-induced apoptosis. Lipopolysaccharide did not significantly inhibit doxorubicin-induced DNA damage detected by single-cell gel electrophoresis (comet) assay. Lipopolysaccharide definitely inhibited the stabilization and nuclear translocation of p53 in doxorubicin-treated RAW 264.7 cells. Lipopolysaccharide, as well as being an inhibitor of p53, abolished doxorubicin-induced apoptosis. Therefore, p53 was suggested to play a pivotal role in the prevention of doxorubicin-induced apoptosis in RAW 264.7 cells by lipopolysaccharide.

引用

页码：373 / 379

页数：7

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