Prussian Blue- and lactate oxidase-based amperometric biosensor for lactic acid

被引：88

作者：

Garjonyte, R

Yigzaw, Y

Meskys, R

Malinauskas, A

Gorton, L

机构：

[1] Inst Chem, LT-2600 Vilnius, Lithuania

[2] Lund Univ, Dept Analyt Chem, S-22100 Lund, Sweden

[3] Inst Biochem, LT-2600 Vilnius, Lithuania

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2001年 / 79卷 / 01期

关键词：

biosensor; Prussian Blue; lactate oxidase; lactic acid; nation; flow-injection analysis;

D O I：

10.1016/S0925-4005(01)00845-0

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The properties of amperometric lactate biosensors based on Prussian Blue (PB) and lactate oxidases (LOX) of different origin (from Pediococcus sp. and Enterococcus sp. RL1) immobilised in Nafion were investigated by flow injection analysis. The operating potential was -50 mV (versus Ag/AgCl, 0.1 M KCl). A linear range in the dependence of current response upon the concentration of lactic acid was up to 0.8 mM. The detection limit of electrodes containing a high amount (6 U) of enzyme was below 1 muM. Operational stability could be maintained by exposing the electrodes to low (20 muM) concentrations of lactic acid. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：33 / 38

页数：6

共 28 条

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