Binding evaluation of isoform 1 from Cratylia mollis lectin to human mammary tissues

被引：38

作者：

Beltrao, EIC

Correia, MTS

Figueredo-Silva, J

Coelho, LCBB ^{[1
]}

机构：

[1] Univ Fed Pernambuco, CCB, Dept Bioquim, BR-50670490 Recife, PE, Brazil

[2] Univ Fed Pernambuco, Dept Patol, CCS, BR-50670490 Recife, PE, Brazil

[3] Univ Fed Pernambuco, Lab Imunopatol Keizo Asami, LIKA, BR-50670490 Recife, PE, Brazil

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1998年 / 74卷 / 03期

关键词：

lectin; isoform; human mammary tissue; infiltrating duct carcinoma; fibrocystic disease; fibroadenoma;

D O I：

10.1007/BF02825961

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The phenomenon of altered carbohydrates in transformed cell surfaces has been studied through histochemical techniques using lectins. Specific binding patterns to normal and transformed mammary tissues were evaluated by Isoform 1 from Cratylia mollis lectin (Cra Iso 1). Protocols using a direct method, incubation of Cra Iso I conjugated to peroxidase (Cra Iso 1-Per) with mammary tissues, followed by diaminobenzidine and hydrogen peroxidase interaction, were performed. Neoplastic tissues, marked by Cra Iso 1, showed a higher intensity of staining than normal ones, in comparison with Canavalia ensiformis lectin, Concanavalin A (Con A), conjugated to peroxidase (Con A-Per). The assay with Cra Iso 1 also indicated a possible utilization of this lectin to characterize normal and transformed mammary cells.

引用

页码：125 / 134

页数：10

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