Purification of a glucose mannose specific lectin, isoform 1, from seeds of Cratylia mollis Mart (Camaratu bean)

被引：119

作者：

Correia, MTS ^{[1
]}

Coelho, LCBB ^{[1
]}

机构：

[1] UNIV FED PERNAMBUCO,CTR CIENCIAS BIOL,DEPT BIOQUIM,BR-50670420 RECIFE,PE,BRAZIL

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1995年 / 55卷 / 03期

关键词：

lectin; isoform; lectin purification; antilectin IgG; antilectin IgG-peroxidase;

D O I：

10.1007/BF02786865

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A quantitatively main molecular form of Cratylia mollis lectin, isoform 1 (iso 1) was purified by affinity chromatography on Sephadex G-75, followed by ion exchange chromatography on CM-cellulose. Another lectin form was identified in the latter step. Iso 1 is specific for glucose/mannose, with a main subunit of 31 kDa mol wt; the native protein is basic (pI 8.5-8.6) and the constituent polypeptides had a pi range of 5.15-7.75. An antibody to the protein was raised in a rabbit, and the conjugate was active in an immunosorbent assay.

引用

页码：261 / 273

页数：13

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