Retrospective an reverse genetics in mice around the world and in Japan

被引：1

作者：

Aizawa, Shinichi ^{[1
]}

机构：

[1] RIKEN, Ctr Dev Biol, Lab Vertebrate Body Plan & Anim Resources & Genet, Chuo Ku, Kobe, Hyogo 6500047, Japan

来源：

DEVELOPMENT GROWTH & DIFFERENTIATION | 2008年 / 50卷

关键词：

cloned animal; ES cell; gene targeting; iPS cell; knockout mouse; teratocarcinoma;

D O I：

10.1111/j.1440-169X.2008.01004.x

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The 2007 Nobel Prize for Physiology or Medicine was awarded to Mario R. Capecchi, Martin J. Evans and Oliver Smithies for their contribution in generating mutant mice by gene targeting in embryonic stem (ES) cells. Although there are many experimental animals, it is yet only in mouse that one can genetically examine functions of genes at will. It was merely a dream in the early 1980s that genetic studies with mutants would one day become a reality in mammals. The story began with tetratocarcinoma/embryonal carcinoma cells. Now, through the successes of cloning in mammals, somatic cells such as our skin cells will shortly be transformed into ES-like (induced pluripotent stem) cells by the proper activation of endogenous genes such as Oct14 and Sox2 with chemicals. How have times changed?

引用

页码：S29 / S34

页数：6

共 32 条

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