Cloning of the Arabidopsis ent-kaurene oxidase gene GA3

被引：186

作者：

Helliwell, CA

Sheldon, CC

Olive, MR

Walker, AR

Zeevaart, JAD

Peacock, WJ

Dennis, ES

机构：

[1] Commonwealth Sci & Ind Res Org, Plant Ind, Canberra, ACT 2601, Australia

[2] Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England

[3] Michigan State Univ, Dept Energy Plant Res Lab, E Lansing, MI 48824 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1998年 / 95卷 / 15期

关键词：

D O I：

10.1073/pnas.95.15.9019

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The ga3 mutant of Arabidopsis is a gibberellin-responsive dwarf. We present data showing that the ga3-1 mutant is deficient in ent-kaurene oxidase activity, the first cytochrome P450-mediated step in the gibberellin biosynthetic pathway. By using a combination of conventional map-based cloning and random sequencing we identified a putative cytochrome P450 gene mapping to the same location as GA3. Relative to the progenitor line, two ga3 mutant alleles contained single base changes generating in-frame stop codons in the predicted amino acid sequence of the P450. A genomic clone spanning the P450 locus complemented the ga3-2 mutant. The deduced GA3 protein defines an additional class of cytochrome P450 enzymes. The GA3 gene was expressed in all tissues examined, RNA abundance being highest in inflorescence tissue.

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页码：9019 / 9024

页数：6

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