Isolation of cDNA clones encoding human histone macroH2A1 subtypes

被引：8

作者：

Lee, YH

Hong, MH

Kim, JW

Hong, YM

Choe, YK

Chang, SY

Lee, KS

Choe, IS

机构：

[1] Korea Res Inst Biosci & Biotechnol, Mol & Cellular Biol Res Grp, Taejon 305600, South Korea

[2] Hanyang Univ, Sch Med, Inst Rheumatol, Seoul 133792, South Korea

[3] Hanyang Univ, Sch Med, Dept Surg, Seoul 133792, South Korea

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1998年 / 1399卷 / 01期

关键词：

macroH2Al subtype; cDNA sequence; tissue distribution; (human);

D O I：

10.1016/S0167-4781(98)00098-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

cDNA clones encoding two different subtypes of histone macroH2A1, macroH2A1.1 and macroH2A1.2, have been isolated from human liver tissue. The open reading frames in the isolated clones predicted proteins comprising 368 and 371 amino acids respectively. Estimated molecular masses of the two proteins were 39.0 kDa and 39.4 kDa. Human histone macroH2A1.1 and macroH2A1.2 showed about 98% identity with their counterparts isolated from rat. The features of the nucleotide sequences of the two macroH2A1 subtypes in human were the same as in the rat system. Northern blot analysis showed that the macroH2A1.1 and macroH2A1.2 subtypes were expressed as mRNA species with a size of 1.5 and 4.4 kb. respectively. They were expressed in all human tissues examined. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：73 / 77

页数：5