Lock-in by molecular multiplication

被引：16

作者：

Braun, D ^{[1
]}

Libchaber, A ^{[1
]}

机构：

[1] Rockefeller Univ, Ctr Studies Phys & Biol, New York, NY 10021 USA

来源：

APPLIED PHYSICS LETTERS | 2003年 / 83卷 / 26期

关键词：

D O I：

10.1063/1.1629782

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

A lock-in amplifier is physically realized at the level of fluorescent dye molecules. It is based on the general property that the emission of a fluorescent dye is the product of quantum efficiency and illumination intensity. For each pixel of a microscopic image, we measure in amplitude and phase an environment property of the dye, such as conformation, membrane voltage, or temperature. This lock-in implementation is highly parallel and reaches the ultimate photon shot noise limit. Using fast temperature oscillations, we apply it to measure the opening/closing kinetics of a molecular beacon (DNA hairpin) at 5 mus resolution. (C) 2003 American Institute of Physics.

引用

页码：5554 / 5556

页数：3

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