Iron deficiency induces a chlorophyll d-binding Pcb antenna system around Photosystem I in Acaryochloris marina

被引:34
作者
Chen, M
Bibby, TS
Nield, J
Larkum, A
Barber, J [1 ]
机构
[1] Univ London Imperial Coll Sci Technol & Med, Fac Life Sci, Div Mol Biosci, London SW7 2AZ, England
[2] Univ Sydney, Sch Biol Sci, Sydney, NSW 2006, Australia
来源
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS | 2005年 / 1708卷 / 03期
基金
英国生物技术与生命科学研究理事会; 澳大利亚研究理事会;
关键词
Acaryochloris; iron stress; photosynthesis; Pcb protein; photosystem; gene transcript level;
D O I
10.1016/j.bbabio.2005.05.007
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The prochlorophyte-like cyanobacterium Acaryochloris marina contains two pcb genes, pcbA and pcbC, which encode chlorophyll (Chl) d-binding antenna proteins PcbA and PcbC, respectively. Using real-time reverse transcriptase polymerase chain reaction (RT-PCR), it is shown that when Acaryochloris cells are grown in an iron-deficient medium, the transcription of the pcbC gene is up-regulated compared to that of pcbA. Biochemical and immunological analyses indicated that under the same iron-deficient conditions, the level of Photosystem I (PSI) decreased compared with that of Photosystem II (PSII). Electron microscopy revealed that concomitant with these changes was the formation of Pcb-PSI supercomplexes which, in their largest form, were composed of 18 Pcb subunits forming a ring around the trimeric PSI reaction centre core. Mass spectrometry indicated that the PcbC protein is the main constituent of this outer PSI antenna system. It is therefore concluded that in Acaryochloris, the PcbC protein forms an antenna for PSI when iron levels become limiting and in this way compensates for the drop in the level of PSI relative to PSII which occurs under these conditions. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:367 / 374
页数:8
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