Analysis of vitamin B12 in food by silica gel 60 TLC and bioautography with vitamin B12-dependent Escherichia coli 215

被引：30

作者：

Tanioka, Yuri ^{[2
]}

Yabuta, Yukinori ^{[1
]}

Miyamoto, Emi ^{[3
]}

Inui, Hiroshi ^{[2
]}

Watanabe, Fumio ^{[1
]}

机构：

[1] Tottori Univ, Fac Agr, Sch Agr Biol & Environm Sci, Tottori 6808553, Japan

[2] Osaka Prefecture Univ, Grad Sch Agr & Biol Sci, Sakai, Osaka 591, Japan

[3] Nagasaki Int Univ, Dept Hlth & Nutr, Sasebo, Japan

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2008年 / 31卷 / 13期

关键词：

bioautography; edible cyanobacteria; Escherichia coli 215; TLC; vitamin B-12;

D O I：

10.1080/10826070802197453

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

To evaluate whether certain foods contain vitamin B-12 or inactive corrinoids, a simple technique, bioautography with vitamin B-12-dependent Escherichia coli mutant after separation of the sample by silica gel 60 thin-layer chromatography, is available. By using the method, vitamin B-12-compounds found in some edible cyanobacteria are readily identified. This bioautography has great advantages (simplicity, speed, and inexpensiveness) for the analysis of vitamin B-12-compounds in food.

引用

页码：1977 / 1985

页数：9

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