NIR-to-NIR Two-Photon Excited CaF2: Tm3+,Yb3+ Nanoparticles: Multifunctional Nanoprobes for Highly Penetrating Fluorescence Bio-Imaging

被引：360

作者：

Dong, Ning-Ning ^{[3
,4
]}

Pedroni, Marco ^{[1
,2
]}

Piccinelli, Fabio ^{[1
,2
]}

Conti, Giamaica ^{[5
]}

Sbarbati, Andrea ^{[5
]}

Enrique Ramirez-Hernandez, Juan

Martinez Maestro, Laura ^{[3
]}

Carmen Iglesias-de la Cruz, Maria ^{[6
]}

Sanz-Rodriguez, Francisco ^{[7
]}

Juarranz, Angeles ^{[7
]}

Chen, Feng ^{[4
]}

Vetrone, Fiorenzo ^{[8
]}

Capobianco, John A. ^{[9
]}

Garcia Sole, Jose ^{[3
]}

Bettinelli, Marco ^{[1
,2
]}

Jaque, Daniel ^{[3
]}

Speghini, Adolfo ^{[1
,2
]}

机构：

[1] Univ Verona, Dipartimento Biotecnol, I-37134 Verona, Italy

[2] UdR Verona, INSTM, I-37134 Verona, Italy

[3] Univ Autonoma Madrid, Fac Ciencias, Dept Fis Mat, Fluorescence Imaging Grp, E-28049 Madrid, Spain

[4] Shandong Univ, State Key Lab Crystal Mat, Sch Phys, Jinan 250100, Peoples R China

[5] Univ Verona, Dipartimento Sci Neurol Neuropsicol Morfol & Moto, I-37134 Verona, Italy

[6] Univ Autonoma Madrid, Fac Med, Dept Fisiol, Madrid 29029, Spain

[7] Univ Autonoma Madrid, Fac Ciencias, Dept Biol, E-28049 Madrid, Spain

[8] Univ Quebec, Inst Natl Rech Sci Energie Mat & Telecommun, Varennes, PQ J3X 1S2, Canada

[9] Concordia Univ, Dept Chem & Biochem, Montreal, PQ H4B 1R6, Canada

来源：

ACS NANO | 2011年 / 5卷 / 11期

基金：

加拿大自然科学与工程研究理事会;

关键词：

multifunctional nanoprobes; fluorescence bio-imaging; nanothermometers; near-infrared upconversion; calcium fluoride; UP-CONVERSION LUMINESCENCE; IN-VIVO; CELLS; NANOCRYSTALS; LIGHT; TM3+;

D O I：

10.1021/nn202490m

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this study, we report on the remarkable two-photon excited fluorescence efficiency in the "biological window" of CaF2:Tm3+,Yb3+ nanoparticles. On the basis of the strong Tm3+ ion emission (at around 800 nm), tissue penetration depths as large as 2 mm have been demonstrated, which are more than 4 times those achievable based on the visible emissions in comparable CaF2:Er3+,Yb3+ nanoparticles. The outstanding penetration depth, together with the fluorescence thermal sensitivity demonstrated here, makes CaF2:Tm3+,Yb3+ nanoparticles ideal candidates as multifunctional nanoprobes for high contrast and highly penetrating in vivo fluorescence imaging applications.

引用

页码：8665 / 8671

页数：7

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