Colorimetric bio-barcode amplification assay for cytokines

被引：109

作者：

Nam, JM

Wise, AR

Groves, JT ^{[1
]}

机构：

[1] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[2] Lawrence Berkeley Lab, Phys Biosci Div, Berkeley, CA 94720 USA

来源：

ANALYTICAL CHEMISTRY | 2005年 / 77卷 / 21期

关键词：

D O I：

10.1021/ac0513764

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The bio-barcode amplification assay has become a powerful tool in detecting tens to hundreds of biological targets such as proteins and nucleic acids in the entire sample. However, current bio-barcode detection schemes still require many experimental steps including microarrayerbased immobilization of oligonucleotides on a glass chip, silver enhancement of immobilized gold nanoparticles on a chip, and light-scattering measurement. Here, we report a colorimetric bio-barcode method that minimizes the above requirements while detecting 30 aM concentrations of cytokines (similar to 3 orders of magnitude more sensitive than conventional nonenzymatic cytokine detection assays). The assay is based on porous microparticles, which enable loading of a large number of barcode DNA per particle, and gold nanoparticle-based colorimetric barcode detection method.

引用

页码：6985 / 6988

页数：4

共 27 条

[11] Multiplexed protein profiling on antibody-based microarrays by rolling circle amplification [J].