Micropropagation of tea (Camellia sinensis (L.) O.!Kuntze) using Thidiazuron

被引：29

作者：

Mondal, TK ^{[1
]}

Bhattacharya, A ^{[1
]}

Sood, A ^{[1
]}

Ahuja, PS ^{[1
]}

机构：

[1] Inst Himalayan Bioresource Technol, Div Biotechnol, Palampur 176061, Himachal Prades, India

来源：

PLANT GROWTH REGULATION | 1998年 / 26卷 / 01期

关键词：

axillary shoot multiplication; Camellia sinensis (L.) O. Kuntze; nodal segments; Thidiazuron; woody plant;

D O I：

10.1023/A:1006019206264

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The effect of thidiazuron (TDZ) on the micropropagation of Camellia sinensis (China hybrid) was compared with that of benzylaminopurine (BAP) using nodal segments from in vitro raised seedlings. Extremely low concentrations of TDZ (1pM-100nM) alone were effective in inducing shoot bud proliferation and maintaining high rates of shoot multiplication on hormone-free media. On the other hand, higher concentrations of BAP (1-10 mu M) and its continued presence were required to initiate and sustain shoot proliferation. While wider ranges of BAP combined favourably with auxins like NAA or IBA, only specific combinations of TDZ and NAA were effective for shoot proliferation. TDZ treated explants yielded healthy shoots, with sturdy leaves, even during the initial stages of growth, whereas, the effect of BAP was cumulative over subcultures in attaining a high proliferative rate.

引用

页码：57 / 61

页数：5