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Monomeric (glycine-proline-hydroxyproline)10 repeat sequence is a partial agonist of the platelet collagen receptor glycoprotein VI
被引:55
作者:
Asselin, J
Knight, CG
Farndale, RW
Barnes, MJ
Watson, SP
机构:
[1] Univ Oxford, Dept Pharmacol, Oxford OX1 3QT, England
[2] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England
关键词:
adhesion molecule;
receptor recognition motif;
tyrosine kinase;
D O I:
10.1042/0264-6021:3390413
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
We have previously reported that a triple-helical, collagen-related peptide (CRP; also known as CRP-XL) containing a glycine-proline-hydroxyproline (GPP*) repeat motif and cross-linked through cysteine residues at its N-terminus and C-terminus is a powerful stimulus of platelet aggregation and secretion through the surface receptor glycoprotein VI (GPVI). The activation of platelets is associated with tyrosine phosphorylation of the tyrosine kinase Syk and phospholipase C gamma 2 (PLC gamma 2). We now report that the non-cross-linked backbone of CRP, monomeric CRP (mCRP), stimulates the tyrosine phosphorylation of Syk and PLC gamma 2 in platelets and induces the weak secretion of [H-3]5-hydroxytryptamine ([H-3]5-HT) and aggregation. The action of mCRP does not seem to be due to spontaneous cross-linking, because alkylation of the cysteine residues leads to an increase in activity. The tripeptide backbone of CRP, GPP(10)(*) (in which P* represents hydroxyproline) also stimulates platelet shape change and the weak tyrosine phosphorylation of Syk and PLC gamma 2, but is unable to induce aggregation or secretion. The monomeric peptides partly inhibit the release of [H-3]S-HT by CRP, suggesting that they are partial agonists of the collagen receptor GPVI. These results demonstrate that GPP* present as a repeat motif is sufficient to activate the platelet collagen receptor GPVI but that the cross-linking of monomers brings about an increase in activity.
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页码:413 / 418
页数:6
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