Antimicrobial peptide resistance mediates resilience of prominent gut commensals during inflammation

被引：308

作者：

Cullen, T. W. ^{[1
,2
]}

Schofield, W. B. ^{[1
,2
]}

Barry, N. A. ^{[1
,2
]}

Putnam, E. E. ^{[1
,2
]}

Rundell, E. A. ^{[1
]}

Trent, M. S. ^{[3
,4
,5
]}

Degnan, P. H. ^{[6
]}

Booth, C. J.

Yu, H. ^{[7
]}

Goodman, A. L. ^{[1
,2
]}

机构：

[1] Yale Univ, Sch Med, Dept Microbial Pathogenesis, New Haven, CT 06520 USA

[2] Yale Univ, Sch Med, Microbial Sci Inst, New Haven, CT 06520 USA

[3] Yale Univ, Comparat Med Sect, Sch Med, New Haven, CT 06520 USA

[4] Univ Texas Austin, Dept Mol Biosci, Austin, TX 78712 USA

[5] Univ Texas Austin, Inst Cell & Mol Biol, Austin, TX 78712 USA

[6] Univ Illinois, Dept Microbiol, Urbana, IL 61801 USA

[7] Univ Hawaii, Canc Epidemiol Program, Ctr Canc, Honolulu, HI 96813 USA

来源：

SCIENCE | 2015年 / 347卷 / 6218期

关键词：

IMMUNE-SYSTEM; LIPID-A; MICROBIOTA; BACTERIAL; MEMBRANE; HOST; COLONIZATION; RESPONSES; DISEASE;

D O I：

10.1126/science.1260580

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Resilience to host inflammation and other perturbations is a fundamental property of gut microbial communities, yet the underlying mechanisms are not well understood. We have found that human gut microbes from all dominant phyla are resistant to high levels of inflammation-associated antimicrobial peptides (AMPs) and have identified a mechanism for lipopolysaccharide (LPS) modification in the phylum Bacteroidetes that increases AMP resistance by four orders of magnitude. Bacteroides thetaiotaomicron mutants that fail to remove a single phosphate group from their LPS were displaced from the microbiota during inflammation triggered by pathogen infection. These findings establish a mechanism that determines the stability of prominent members of a healthy microbiota during perturbation.

引用

页码：170 / 175

页数：6

共 23 条

[1]

Gut Immune Maturation Depends on Colonization with a Host-Specific Microbiota [J].

Chung, Hachung ;

Pamp, Suenje J. ;

Hill, Jonathan A. ;

Surana, Neeraj K. ;

Edelman, Sanna M. ;

Troy, Erin B. ;

Reading, Nicola C. ;

Villablanca, Eduardo J. ;

Wang, Sen ;

Mora, Jorge R. ;

Umesaki, Yoshinori ;

Mathis, Diane ;

Benoist, Christophe ;

Relman, David A. ;

Kasper, Dennis L. .

CELL, 2012, 149 (07) :1578-1593

[2]

The Lipid A Phosphate Position Determines Differential Host Toll-Like Receptor 4 Responses to Phylogenetically Related Symbiotic and Pathogenic Bacteria [J].

Coats, Stephen R. ;

Berezow, Alex B. ;

To, Thao T. ;

Jain, Sumita ;

Bainbridge, Brian W. ;

Banani, Karim P. ;

Darveau, Richard P. .

INFECTION AND IMMUNITY, 2011, 79 (01) :203-210

[3]

Porphyromonas gingivalis Resistance to Polymyxin B Is Determined by the Lipid A 4′-Phosphatase, PGN_0524 [J].

Coats, Stephen R. ;

To, Thao T. ;

Jain, Sumita ;

Braham, Pamela H. ;

Darveau, Richard P. .

INTERNATIONAL JOURNAL OF ORAL SCIENCE, 2009, 1 (03) :126-135

[4]

Helicobacter pylori versus the Host: Remodeling of the Bacterial Outer Membrane Is Required for Survival in the Gastric Mucosa [J].

Cullen, Thomas W. ;

Giles, David K. ;

Wolf, Lindsey N. ;

Ecobichon, Chantal ;

Boneca, Ivo G. ;

Trent, M. Stephen .

PLOS PATHOGENS, 2011, 7 (12)

[5]

A link between the assembly of flagella and lipooligosaccharide of the Gram-negative bacterium Campylobacter jejuni [J].

Cullen, Thomas W. ;

Trent, M. Stephen .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (11) :5160-5165

[6]

The Long-Term Stability of the Human Gut Microbiota [J].

Faith, Jeremiah J. ;

Guruge, Janaki L. ;

Charbonneau, Mark ;

Subramanian, Sathish ;

Seedorf, Henning ;

Goodman, Andrew L. ;

Clemente, Jose C. ;

Knight, Rob ;

Heath, Andrew C. ;

Leibel, Rudolph L. ;

Rosenbaum, Michael ;

Gordon, Jeffrey I. .

SCIENCE, 2013, 341 (6141) :44-+

[7]

Identifying Genetic Determinants Needed to Establish a Human Gut Symbiont in Its Habitat [J].

Goodman, Andrew L. ;

McNulty, Nathan P. ;

Zhao, Yue ;

Leip, Douglas ;

Mitra, Robi D. ;

Lozupone, Catherine A. ;

Knight, Rob ;

Gordon, Jeffrey I. .

CELL HOST & MICROBE, 2009, 6 (03) :279-289

[8]

Interactions Between the Microbiota and the Immune System [J].

Hooper, Lora V. ;

Littman, Dan R. ;

Macpherson, Andrew J. .

SCIENCE, 2012, 336 (6086) :1268-1273

[9]

MULTIPLE GENES FOR MEMBRANE-BOUND PHOSPHATASES IN ESCHERICHIA-COLI AND THEIR ACTION ON PHOSPHOLIPID PRECURSORS [J].

ICHO, T ;

RAETZ, CRH .

JOURNAL OF BACTERIOLOGY, 1983, 153 (02) :722-730

[10]

A Francisella mutant in lipid a carbohydrate modification elicits protective immunity [J].

Kanistanon, Duangjit ;

Hajjar, Adeline M. ;

Pelletier, Mark R. ;

Gallagher, Larry A. ;

Kalhorn, Thomas ;

Shaffer, Scott A. ;

Goodlett, David R. ;

Rohmer, Laurence ;

Brittnacher, Mitchell J. ;

Skerrett, Shawn J. ;

Ernst, Robert K. .

PLOS PATHOGENS, 2008, 4 (02)

← 1 2 3 →