MacroH2AI knockdown effects on the Peg3 imprinted domain

被引：7

作者：

Choo, Jung Ha ^{[1
,2
]}

Do Kim, Jeong ^{[1
]}

Kim, Joomyeong ^{[1
]}

机构：

[1] Louisiana State Univ, Dept Biol Sci, Baton Rouge, LA 70803 USA

[2] Korea Adv Inst Sci & Technol, Dept Biol Sci, Taejon 305701, South Korea

来源：

BMC GENOMICS | 2007年 / 8卷 / 1期

关键词：

D O I：

10.1186/1471-2164-8-479

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Background: MacroH2AI is a histone variant that is closely associated with the repressed regions of chromosomes. A recent study revealed that this histone variant is highly enriched in the inactive alleles of Imprinting Control Regions (ICRs). Results: The current study investigates the potential roles of macroH2AI in genomic imprinting by lowering the cellular levels of the macroH2AI protein. RNAi- based macroH2AI knockdown experiments in Neuro2A cells changed the expression levels of a subset of genes, including Peg3 and Usp29 of the Peg3 domain. The expression of these genes was down-regulated, rather than up-regulated, in response to reduced protein levels of the potential repressor macroH2AI. This down-regulation was not accompanied with changes in the DNA methylation status of the Peg3 domain. Conclusion: MacroH2AI may not function as a transcriptional repressor for this domain, but that macroH2AI may participate in the heterochromatin formation with functions yet to be discovered.

引用

页数：8

共 24 条

[1] The histone variant macroH2A interferes with transcription factor binding and SWI/SNF nucleosome remodeling [J].

Angelov, D ;

Molla, A ;

Perche, PY ;

Hans, F ;

Côté, J ;

Khochbin, S ;

Bouvet, P ;

Dimitrov, S .

MOLECULAR CELL, 2003, 11 (04) :1033-1041

[2] Mechanisms of genomic imprinting [J].

Brannan, CI ;

Bartolomei, MS .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 1999, 9 (02) :164-170

[3] Upstream stimulatory factor 2 stimulates transcription through an initiator element in the mouse cytochrome c oxidase subunit Vb promoter [J].

Breen, GAM ;

Jordan, EM .

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 2000, 1517 (01) :119-127

[4] Histone variant macroH2A contains two distinct macrochromatin domains capable of directing macroH2A to the inactive X chromosome [J].

Chadwick, BP ;

Valley, CM ;

Willard, HF .

NUCLEIC ACIDS RESEARCH, 2001, 29 (13) :2699-2705

[5] Histone H2A variants and the inactive X chromosome: identification of a second macroH2A variant [J].

Chadwick, BP ;

Willard, HF .

HUMAN MOLECULAR GENETICS, 2001, 10 (10) :1101-1113

[6] Developmental changes in histone macroH2A1-mediated gene regulation [J].

Changolkar, Lakshmi N. ;

Costanzi, Carl ;

Leu, N. Adrian ;

Chen, Dannee ;

McLaughlin, K. John ;

Pehrson, John R. .

MOLECULAR AND CELLULAR BIOLOGY, 2007, 27 (07) :2758-2764

[7] Allele-specific deposition of macroH2A1 in imprinting control regions [J].

Choo, JH ;

Kim, JD ;

Chung, JH ;

Stubbs, L ;

Kim, J .

HUMAN MOLECULAR GENETICS, 2006, 15 (05) :717-724

[8]

Costanzi C, 2000, DEVELOPMENT, V127, P2283

[9] Histone macroH2A1 is concentrated in the inactive X chromosome of female mammals [J].

Costanzi, C ;

Pehrson, JR .

NATURE, 1998, 393 (6685) :599-601

[10] Identification of clustered YY1 binding sites in imprinting control regions [J].

Do Kim, Jeong ;

Hinz, Angela K. ;

Bergmann, Anne ;

Huang, Jennifer M. ;

Ovcharenko, Ivan ;

Stubbs, Lisa ;

Kim, Joomyeong .

GENOME RESEARCH, 2006, 16 (07) :901-911

← 1 2 3 →