Immunomodulation of phytohormones and functional proteins in plant cells

被引：32

作者：

Conrad, U ^{[1
]}

Manteuffel, R ^{[1
]}

机构：

[1] Inst Pflanzengent & Kulturpflanzenforsch Gatersle, D-06466 Gatersleben, Germany

来源：

TRENDS IN PLANT SCIENCE | 2001年 / 6卷 / 09期

关键词：

D O I：

10.1016/S1360-1385(01)02043-X

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Recombinant antibodies expressed ectopically in plant cells recognize their corresponding antigens and can therefore bind specifically to phytohormones and proteins in vivo. The generation of antibody-antigen complexes interferes with the functions of the targets and affects the phenotype of transgenic plants. Recombinant antibodies can accumulate in different cell compartments and organs of transgenic plants at different stages of development. High levels of expression of specific, high-affinity antibodies are required for immunomodulation. Here, we discuss several models and examples of the antibody-mediated modulation of phytohormone and protein functions in terms of their potential for plant research.

引用

页码：399 / 402

页数：4

共 41 条

[11] EXPRESSION OF ENGINEERED ANTIBODIES IN PLANT-CELLS [J].

CONRAD, U ;

FIEDLER, U .

PLANT MOLECULAR BIOLOGY, 1994, 26 (04) :1023-1030

[12] Compartment-specific accumulation of recombinant immunoglobulins in plant cells: an essential tool for antibody production and immunomodulation of physiological functions and pathogen activity [J].

Conrad, U ;

Fiedler, U .

PLANT MOLECULAR BIOLOGY, 1998, 38 (1-2) :101-109

[13] High level accumulation of single-chain variable fragments in the cytosol of transgenic Petunia hybrida [J].

De Jaeger, G ;

Buys, E ;

Eeckhout, D ;

De Wilde, C ;

Jacobs, A ;

Kapila, J ;

Angenon, G ;

Van Montagu, M ;

Gerats, T ;

Depicker, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 259 (1-2) :426-434

[14] The plantibody approach: expression of antibody genes in plants to modulate plant metabolism or to obtain pathogen resistance [J].

De Jaeger, G ;

De Wilde, C ;

Eeckhout, D ;

Fiers, E ;

Depicker, A .

PLANT MOLECULAR BIOLOGY, 2000, 43 (04) :419-428

[15] Antibody arrays for high-throughput screening of antibody-antigen interactions [J].

de Wildt, RMT ;

Mundy, CR ;

Gorick, BD ;

Tomlinson, IM .

NATURE BIOTECHNOLOGY, 2000, 18 (09) :989-994

[16] Isolation and characterization of recombinant antibody fragments against CDC2a from Arabidopsis thaliana [J].

Eeckhout, D ;

Fiers, E ;

Sienaert, R ;

Snoeck, V ;

Depicker, A ;

De Jaeger, G .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (23) :6775-6783

[17] HIGH-LEVEL PRODUCTION AND LONG-TERM STORAGE OF ENGINEERED ANTIBODIES IN TRANSGENIC TOBACCO SEEDS [J].

FIEDLER, U ;

CONRAD, U .

BIO-TECHNOLOGY, 1995, 13 (10) :1090-1093

[18]

HAMILTON AJ, 1995, CURR TOP MICROBIOL, V197, P77

[19] PRODUCTION OF ANTIBODIES IN TRANSGENIC PLANTS [J].

HIATT, A ;

CAFFERKEY, R ;

BOWDISH, K .

NATURE, 1989, 342 (6245) :76-78

[20] Natural and designer binding sites made by phage display technology [J].

Hoogenboom, HR ;

Chames, P .

IMMUNOLOGY TODAY, 2000, 21 (08) :371-378

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