Cloning, sequencing and expression of the cDNA of endoxylanase B from Penicillium purpurogenum

被引：21

作者：

Diaz, R

Sapag, A

Peirano, A

Steiner, J

Eyzaguirre, J

机构：

[1] PONTIFICIA UNIV CATOLICA CHILE, LAB BIOQUIM, SANTIAGO, CHILE

[2] UNIV CHILE, FAC CIENCIAS QUIM & FARMACEUT, DEPT BIOQUIM & BIOL MOL, SANTIAGO, CHILE

来源：

GENE | 1997年 / 187卷 / 02期

关键词：

endoxylanase; P-purpurogenum; nucleotide sequence; glycosyl hydrolase; PCR; sequence alignment; homology comparison; codon usage;

D O I：

10.1016/S0378-1119(96)00762-7

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The cDNA, for xylanase B from Penicillium purpurogenum was cloned and sequenced. This DNA encodes a protein of 208 amino acids which is expected to yield a protein of 183 residues upon processing of the N terminus. The sequence of the predicted protein is very similar to that of 40 other xylanase domains which belong to family G of cellulases/xylanases (73-21% identity).

引用

收藏

页码：247 / 251

页数：5

相关论文

共 39 条

[11] DOMAINS IN MICROBIAL BETA-1,4-GLYCANASES - SEQUENCE CONSERVATION, FUNCTION, AND ENZYME FAMILIES [J].

GILKES, NR ;

HENRISSAT, B ;

KILBURN, DG ;

MILLER, RC ;

WARREN, RAJ .

MICROBIOLOGICAL REVIEWS, 1991, 55 (02) :303-315

[12] ISOLATION AND CHARACTERIZATION OF A 1,4-BETA-ENDOXYLANASE GENE OF A-AWAMORI [J].

HESSING, JGM ;

VANROTTERDAM, C ;

VERBAKEL, JMA ;

ROZA, M ;

MAAT, J ;

VANGORCOM, RFM ;

VANDENHONDEL, CAMJJ .

CURRENT GENETICS, 1994, 26 (03) :228-232

[13] CLUSTAL - A PACKAGE FOR PERFORMING MULTIPLE SEQUENCE ALIGNMENT ON A MICROCOMPUTER [J].

HIGGINS, DG ;

SHARP, PM .

GENE, 1988, 73 (01) :237-244

[14] CHARACTERIZATION AND SEQUENCE OF A THERMOMONOSPORA-FUSCA XYLANASE [J].

IRWIN, D ;

JUNG, ED ;

WILSON, DB .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (03) :763-770

[15] CLONING AND SEQUENCING OF THE XYNC GENE ENCODING ACID XYLANASE OF ASPERGILLUS-KAWACHII [J].

ITO, K ;

IWASHITA, K ;

IWANO, K .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1992, 56 (08) :1338-1340

[16] CLONING OF THE XYNNB GENE ENCODING XYLANASE-B FROM ASPERGILLUS-NIGER AND ITS EXPRESSION IN ASPERGILLUS-KAWACHII [J].

KINOSHITA, K ;

TAKANO, M ;

KOSEKI, T ;

ITO, K ;

IWANO, K .

JOURNAL OF FERMENTATION AND BIOENGINEERING, 1995, 79 (05) :422-428

[17] SITE-DIRECTED MUTAGENESIS AT ASPARTATE AND GLUTAMATE RESIDUES OF XYLANASE FROM BACILLUS-PUMILUS [J].

KO, EP ;

AKATSUKA, H ;

MORIYAMA, H ;

SHINMYO, A ;

HATA, Y ;

KATSUBE, Y ;

URABE, I ;

OKADA, H .

BIOCHEMICAL JOURNAL, 1992, 288 :117-121

[18]

KOERNER TJ, 1991, METHOD ENZYMOL, V194, P477

[19] CLONING, SEQUENCING, AND REGULATION OF A XYLANASE GENE FROM THE FUNGUS AUREOBASIDIUM-PULLULANS Y-2311-1 [J].

LI, XL ;

LJUNGDAHL, LG .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1994, 60 (09) :3160-3166

[20]

MAAT J, 1992, PROGR BIOTECHNOL, V7, P349

← 1 2 3 4 →