Analysis of Mason-Pfizer monkey virus Gag particles by scanning transmission electron microscopy

被引：22

作者：

Parker, SD

Wall, JS

Hunter, E

机构：

[1] Univ Alabama, Dept Microbiol, Birmingham, AL 35294 USA

[2] Univ Alabama, Dept Med, Div Infect Dis, Birmingham, AL 35294 USA

[3] Brookhaven Natl Lab, Dept Biol, Upton, NY 11973 USA

来源：

JOURNAL OF VIROLOGY | 2001年 / 75卷 / 19期

关键词：

D O I：

10.1128/JVI.75.19.9543-9548.2001

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Mason-Pfizer monkey virus immature capsids selected from the cytoplasm of baculovirus-infected cells were imaged by scanning transmission electron microscopy. The masses of individual selected Gag particles were measured, and the average mass corresponded to 1,900 to 2,100 Gag polyproteins per particle. A large variation in Gag particle mass was observed within each population measured.

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页码：9543 / 9548

页数：6

相关论文

共 33 条

[1] Organization of HIV-1 capsid proteins on a lipid monolayer [J].

Barklis, E ;

McDermott, J ;

Wilkens, S ;

Fuller, S ;

Thompson, D .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (13) :7177-7180

[2] Structural analysis of membrane-bound retrovirus capsid proteins [J].

Barklis, E ;

McDermott, J ;

Wilkens, S ;

Schabtach, E ;

Schmid, MF ;

Fuller, S ;

Karanjia, S ;

Love, Z ;

Jones, R ;

Rui, YJ ;

Zhao, XM ;

Thompson, D .

EMBO JOURNAL, 1997, 16 (06) :1199-1213

[3]

Berkowitz R, 1996, CURR TOP MICROBIOL, V214, P177

[4] LOW MOLECULAR WEIGHT RNAS OF ROUS SARCOMA VIRUS .1. 4-S RNA [J].

BISHOP, JM ;

LEVINSON, WE ;

QUINTRELL, N ;

SULLIVAN, D ;

FANSHIER, L ;

JACKSON, J .

VIROLOGY, 1970, 42 (01) :182-+

[5] The three-dimensional solution structure of the matrix protein from the type D retrovirus, the Mason-Pfizer monkey virus, and implications for the morphology of retroviral assembly [J].

Conte, MR ;

Klikova, M ;

Hunter, E ;

Ruml, T ;

Matthews, S .

EMBO JOURNAL, 1997, 16 (19) :5819-5826

[6] STUDIES ON RNA FROM AVIAN MYELOBLASTOSIS VIRUS [J].

ERIKSON, RL .

VIROLOGY, 1969, 37 (01) :124-+

[7] CHARACTERIZATION OF LOW-MOLECULAR-WEIGHT RNAS ASSOCIATED WITH 70S RNA OF ROUS-SARCOMA VIRUS [J].

FARAS, AJ ;

GARAPIN, AC ;

LEVINSON, WE ;

BISHOP, JM ;

GOODMAN, HM .

JOURNAL OF VIROLOGY, 1973, 12 (02) :334-342

[8] Molecular modelling study of HIV p17gag (MA) protein shell utilising data from electron microscopy and X-ray crystallography [J].

Forster, MJ ;

Mulloy, B ;

Nermut, MV .

JOURNAL OF MOLECULAR BIOLOGY, 2000, 298 (05) :841-857

[9] Cryo-electron microscopy reveals ordered domains in the immature HIV-1 particle [J].

Fuller, SD ;

Wilk, T ;

Gowen, BE ;

Krausslich, HG ;

Vogt, VM .

CURRENT BIOLOGY, 1997, 7 (10) :729-738

[10] Crystal structure of human cyclophilin A bound to the amino-terminal domain of HIV-1 capsid [J].

Gamble, TR ;

Vajdos, FF ;

Yoo, SH ;

Worthylake, DK ;

Houseweart, M ;

Sundquist, WI ;

Hill, CP .

CELL, 1996, 87 (07) :1285-1294

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