Rapid extraction of fungal DNA from clinical samples for PCR amplification

被引：13

作者：

Velegraki, A

Kambouris, M

Kostourou, A

Chalevelakis, G

Legakis, NJ

机构：

[1] Univ Athens, Sch Med, Dept Microbiol, GR-11527 Athens, Greece

[2] Evangelismos Gen Hosp, Propaedeut Clin, Dept Internal Med 2, Athens, Greece

[3] Evangelismos Gen Hosp, Dept Internal Med 5, Athens, Greece

来源：

MEDICAL MYCOLOGY | 1999年 / 37卷 / 01期

关键词：

hexadecyltrimethylammonium bromide; rRNA; PCR; fungal DNA;

D O I：

暂无

中图分类号：

R51 [传染病];

学科分类号：

100401 [流行病与卫生统计学];

摘要：

A hexadecyltrimethylammonium bromide (CTAB) method for isolating fungal DNA from clinical samples, suitable for PCR amplification is described. Yeast and filamentous fungi DNA from clinical samples was amplified with primers complementary to the genes coding for rRNA, amplifying a 105 bp fragment and internal transcribed spacer primers amplifying fragments between 242 and 622 bp. The level of sensitivity was 10 +/- 5 yeast and 28 Aspergillus fumigatus CFU ml(-1) of biological fluid.

引用

页码：69 / 73

页数：5

共 22 条

[1]

[Anonymous], [No title captured]

[2]

RAPID AND SIMPLE METHOD FOR PURIFICATION OF NUCLEIC-ACIDS [J].