Some histochemical characteristics of the mucous microenvironment in four salmonids with different susceptibilities to gyrodactylid infections

Skin mucous cells and mucus from four salmonids (rainbow trout, brown trout, Conon salmon, Iijoki salmon) with different susceptibilities to infection with Gyrodactylus derjavini and G. salaris were partly characterized by cytochemistry, immunoblotting and immunocytochemistry. Mucous cell densities in various fin types were partly correlated with resistance to infection with G. derjavini but not with G. salaris. Lectin binding assays indicated slight differences in carbohydrate composition of mucus from the four salmonids but serum antigens specific for salmonids were found in mucus from all of them. Antisera against salmon immunoglobulin and rainbow trout complement factor C3 reacted with mucus from all of the salmonids but not with mucus from phylogenetically unrelated fish. Antisera raised against ACTH reacted with mucus components from rainbow trout, brown trout and Conon salmon but not with mucus from Iijoki salmon. These findings are discussed in relation to the host specificity of ectoparasites and susceptibility to infection.