Activation of the anaerobic ribonucleotide reductase by S-adenosylmethionine

被引：24

作者：

Gambarelli, S

Luttringer, F

Padovani, D

Mulliez, E

Fontecave, M

机构：

[1] CEA, SCIB, DRFMC, Serv Chim Inorgan & Biol, F-38054 Grenoble, France

[2] Univ Grenoble 1, CNRS, DRDC, Lab Chim & Biochim,Ctr Redox Biol,UMR 5047, F-38054 Grenoble, France

来源：

CHEMBIOCHEM | 2005年 / 6卷 / 11期

关键词：

D O I：

10.1002/cbic.200500182

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

HYSCORE experiments with class III ribonucleotide reductase (RNR) provide a clear signature of 5-adenosylmethionine (SAM) bound to the (4Fe-4S) cluster of the activating enzyme β. Experiments with 2H-labeled RNR α demonstrate that, under reducing conditions, the SAM-derived adenosyl radical is the direct precursor of the key glycyl radical found in the active enzyme. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：1960 / 1962

页数：3

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