Development of wide-pore CLEAR supports for applications involving biological macromolecules

被引：6

作者：

Cederfur, J ^{[1
]}

Kempe, M ^{[1
]}

机构：

[1] Univ Lund, Dept Pure & Appl Biochem, SE-22100 Lund, Sweden

来源：

POLYMER BULLETIN | 2001年 / 46卷 / 05期

关键词：

D O I：

10.1007/s002890170046

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The CLEAR supports were originally developed for solid-phase synthesis. In this study, two wide-pore versions of the resins were prepared by radical suspension polymerization using toluene as the porogen. Trimethylolpropane ethoxylate (14/3 EO/OH) triacrylate was used as the cross-linking agent. Resins of two different porosities were synthesized. The permeability to biological macromolecules was investigated by size-exclusion chromatography, using the resin beads as the stationary phase. One of the resins was able to retard thyroglobulin (669 kDa) while the other, prepared with a lower amount of porogen, showed a lower fractionation range (1-60 kDa).

引用

页码：381 / 387

页数：7

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