A microwave-based method for nucleic acid isolation from environmental samples

被引：96

作者：

Orsini, M ^{[1
]}

Romano-Spica, V ^{[1
]}

机构：

[1] Univ Cattolica Sacro Cuore, Sch Med, Inst Hyg, I-00168 Rome, Italy

来源：

LETTERS IN APPLIED MICROBIOLOGY | 2001年 / 33卷 / 01期

关键词：

D O I：

10.1046/j.1472-765X.2001.00938.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Aims: A simple and rapid method was described for DNA isolation directly from activated sludge or other environmental sources, including soil and sediments. Methods and Results: The present method is based on microwave thermal shock and provides DNA suitable for further analysis. It is also effective for RNA extraction. Conclusions: The protocol is effective, easy, fast and does not require the use of expensive equipment or reagents. Significance and Impact of the Study: The described method can be applied to difficult substrates in environmental microbiology studies.

引用

页码：17 / 20

页数：4