Identification of septins in neurofibrillary tangles in Alzheimer's disease

被引:130
作者
Kinoshita, A
Kinoshita, M
Akiyama, H
Tomimoto, H
Akiguchi, I
Kumar, S
Noda, M
Kimura, J
机构
[1] Kyoto Univ, Grad Sch Med, Dept Mol Oncol, Sakyo Ku, Kyoto 6068501, Japan
[2] Kyoto Univ, Grad Sch Med, Dept Neurol, Sakyo Ku, Kyoto 6068501, Japan
[3] Tokyo Inst Psychiat, Dept Neuropathol, Tokyo, Japan
[4] Inst Med & Vet Sci, Hanson Ctr Canc Res, Adelaide, SA, Australia
关键词
D O I
10.1016/S0002-9440(10)65743-4
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Septins are evolutionarily conserved cytoskeletal GTPases that can form heteropolymer complexes involved hi cytokinesis and other cellular processes. We detected expression of the human septin genes Nedd5, H5, Diff6, and hCDC10 in postmortem brain tissues using the reverse transcription-coupled polymerase chain reaction and their products by immunoblot analysis, Four antibodies directed against three septins, Nedd5, H5, and Diff6, consistently labeled neurofibrillary tangles, neuropil threads, and dystrophic neurites in the senile plaques in brains affected by Alzheimer's disease but did not label obvious structures in young control brains. Immunoelectron microscopy revealed that Nedd5 localized to the paired helical filaments, Pre-tangles, the precursory granular deposits that accumulate in the neuronal cytoplasm, also were labeled with the antibodies. These findings suggest that at least the three septins are associated with tau-based paired helical filament core, and may contribute to the formation of neurofibrillary tangle as integral constitutents of paired helical filaments.
引用
收藏
页码:1551 / 1560
页数:10
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