Systematic investigation of the Escherichia coli metabolome for the biosynthetic origin of an isocyanide carbon atom

被引：37

作者：

Brady, SF ^{[1
]}

Clardy, J ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 43期

关键词：

biosynthesis; DNA; heterologous expression; isocyanides; natural products;

D O I：

10.1002/anie.200501942

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Feeding studies were conducted in which E. coil metabolism was controlled through the systematic use of strains that carry mutations in primary metabolic pathways, thus allowing the origin of the isocyanide carbon atom in antibiotic 1 to be determined. This carbon atom was shown to be derived from the C2 atom of ribulose-5P, or a tautomerically equivalent sugar (see scheme; isnA and isnB are the genes responsible for the biosynthetic pathway of 1). © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：7045 / 7048

页数：4

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