Systemic signaling of the plant nitrogen status triggers specific transcriptome responses depending on the nitrogen source in Medicago truncatula

被引:114
作者
Ruffel, Sandrine [1 ]
Freixes, Sandra [2 ]
Balzergue, Sandrine [3 ]
Tillard, Pascal [1 ]
Jeudy, Christian [2 ]
Martin-Magniette, Marie Laure [3 ,6 ]
van der Merwe, Margaretha J. [4 ]
Kakar, Klementina [4 ]
Gouzy, Jerome [5 ]
Fernie, Alisdair R. [4 ]
Udvardi, Michael [4 ]
Salon, Christophe [2 ]
Gojon, Alain [1 ]
Lepetit, Marc [1 ]
机构
[1] INRA, CNRS Sup, Agro UM2,Inst Biol Integrat Plantes, UMR Biochim & Physiol Mol Plantes 5004, F-34060 Montpellier, France
[2] INRA, UMR, Unite Genet & Ecophysiol Legumineuses, F-21065 Dijon, France
[3] INRA, CNRS, UMR 1165, UEVE 8114,Unite Rech Genom Vegetale, F-91057 Evry, France
[4] Max Planck Inst Mol Pflanzenphys, D-14476 Potsdam, Germany
[5] INRA, CNRS, UMR 441 2594, Lab Interact Plantes Microorgan, F-31326 Castanet Tolosan, France
[6] INRA, UMR AgroParisTech MIA 518, F-75231 Paris, France
关键词
D O I
10.1104/pp.107.115667
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Legumes can acquire nitrogen (N) from NO3-, NH4+, and N-2 (through symbiosis with Rhizobium bacteria); however, the mechanisms by which uptake and assimilation of these N forms are coordinately regulated to match the N demand of the plant are currently unknown. Here, we find by use of the split-root approach in Medicago truncatula plants that NO3- uptake, NH4+ uptake, and N-2 fixation are under general control by systemic signaling of plant N status. Indeed, irrespective of the nature of the N source, N acquisition by one side of the root system is repressed by high N supply to the other side. Transcriptome analysis facilitated the identification of over 3,000 genes that were regulated by systemic signaling of the plant N status. However, detailed scrutiny of the data revealed that the observation of differential gene expression was highly dependent on the N source. Localized N starvation results, in the unstarved roots of the same plant, in a strong compensatory up-regulation of NO3- uptake but not of either NH4+ uptake or N-2 fixation. This indicates that the three N acquisition pathways do not always respond similarly to a change in plant N status. When taken together, these data indicate that although systemic signals of N status control root N acquisition, the regulatory gene networks targeted by these signals, as well as the functional response of the N acquisition systems, are predominantly determined by the nature of the N source.
引用
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页码:2020 / 2035
页数:16
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