Optimization of feed rate profile for the monoclonal antibody production

被引:13
作者
Lee, JH [3 ]
Lim, HC
Yoo, YJ
Park, YH
机构
[1] Univ Calif Irvine, Dept Chem & Biochem Engn, Irvine, CA 92697 USA
[2] Seoul Natl Univ, Dept Chem Engn, Seoul 151840, South Korea
[3] Korea Res Inst Biosci & Biotechnol, Bioproc Res Div, Taejon 305600, South Korea
关键词
D O I
10.1007/s004490050572
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The optimal control algorithm to calculate the optimal feed rate profile of nutrient solution containing two limiting nutrients was proposed. Different from other conventional optimization methods, the proposed algorithm calculated the optimal control profiles for different initial and feed conditions. The singular optimal control algorithm, dynamic programming, and nonsingular transformation algorithm were used for the optimization of simple problems of the 4th order and the performances were compared. With the proposed transformation algorithm, the final MAb concentration increased and the CPU time decreased. For the different initial glucose and glutamine conditions, the optimal control profiles were calculated with the proposed transformation algorithm. As the initial glutamine concentration increased, the final MAb concentration also increased due to the cell viability increase. This was also applied to the different feed compositions. When the glutamine concentration was increased in the feed stream, the final MAb concentration also increased.
引用
收藏
页码:137 / 146
页数:10
相关论文
共 15 条
[1]   SIMULATION OF ANIMAL-CELL METABOLISM [J].
BARFORD, JP ;
PHILLIPS, PJ ;
HARBOUR, C .
CYTOTECHNOLOGY, 1992, 10 (01) :63-74
[2]  
BARFORD JP, 1992, ANIMAL CELL TECHNOLOGY : BASIC & APPLIED ASPECTS, VOL 4, P397
[3]   A STRUCTURED KINETIC MODELING FRAMEWORK FOR THE DYNAMICS OF HYBRIDOMA GROWTH AND MONOCLONAL-ANTIBODY PRODUCTION IN CONTINUOUS SUSPENSION-CULTURES [J].
BATT, BC ;
KOMPALA, DS .
BIOTECHNOLOGY AND BIOENGINEERING, 1989, 34 (04) :515-531
[4]   IN PURSUIT OF THE OPTIMAL FED-BATCH PROCESS FOR MONOCLONAL-ANTIBODY PRODUCTION [J].
BIBILA, TA ;
ROBINSON, DK .
BIOTECHNOLOGY PROGRESS, 1995, 11 (01) :1-13
[5]  
DETREMBLAY M, 1992, BIOPROCESS ENG, V7, P229
[6]   AN EVALUATION OF FED-BATCH CULTIVATION METHODS FOR MAMMALIAN-CELLS BASED ON MODEL SIMULATIONS [J].
HANSEN, HA ;
MADSEN, NM ;
EMBORG, C .
BIOPROCESS ENGINEERING, 1993, 9 (05) :205-213
[7]   REPEATED FED-BATCH CULTURE OF HYBRIDOMA CELLS IN NUTRIENT-FORTIFIED HIGH-DENSITY MEDIUM [J].
JO, EC ;
PARK, HJ ;
KIM, DI ;
MOON, HM .
BIOTECHNOLOGY AND BIOENGINEERING, 1993, 42 (10) :1229-1237
[8]  
Lee JH, 1997, BIOTECHNOL BIOENG, V56, P697, DOI 10.1002/(SICI)1097-0290(19971220)56:6&lt
[9]  
697::AID-BIT13&gt
[10]  
3.0.CO