共 43 条
New DNA viruses identified in patients with acute viral infection syndrome
被引:277
作者:

Jones, MS
论文数: 0 引用数: 0
h-index: 0
机构: Blood Syst Res Inst, San Francisco, CA 94118 USA

Kapoor, A
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h-index: 0
机构: Blood Syst Res Inst, San Francisco, CA 94118 USA

Lukashov, VV
论文数: 0 引用数: 0
h-index: 0
机构: Blood Syst Res Inst, San Francisco, CA 94118 USA

论文数: 引用数:
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机构:

Hecht, F
论文数: 0 引用数: 0
h-index: 0
机构: Blood Syst Res Inst, San Francisco, CA 94118 USA

Delwart, E
论文数: 0 引用数: 0
h-index: 0
机构: Blood Syst Res Inst, San Francisco, CA 94118 USA
机构:
[1] Blood Syst Res Inst, San Francisco, CA 94118 USA
[2] Univ Calif San Francisco, Dept Med, San Francisco, CA 94118 USA
[3] Univ Amsterdam, Acad Med Ctr, Dept Human Retrovirol, NL-1105 AZ Amsterdam, Netherlands
[4] Russian Acad Med Sci, DI Ivanovskii Virol Inst, Moscow 123098, Russia
[5] Univ Edinburgh, Ctr Infect Dis, Summerhall EH9 1AJ, Scotland
[6] San Francisco Gen Hosp, Posit Hlth Program HIV, San Francisco, CA 94110 USA
关键词:
D O I:
10.1128/JVI.79.13.8230-8236.2005
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
A sequence-independent PCR amplification method was used to identify viral nucleic acids in the plasma samples of 25 individuals presenting with symptoms of acute viral infection following high-risk behavior for human immunodeficiency virus type 1 transmission. GB virus C/hepatitis G virus was identified in three individuals and hepatitis B virus in one individual. Three previously undescribed DNA viruses were also detected, a parvovirus and two viruses related to TT virus (TTV). Nucleic acids in human plasma that were distantly related to bacterial sequences or with no detectable similarities to known sequences were also found. Nearly complete viral genome sequencing and phylogenetic analysis confirmed the presence of a new parvovirus distinct from known human and animal parvoviruses and of two related TTV-like viruses highly divergent from both the TTV and TTV-like minivirus groups. The detection of two previously undescribed viral species in a small group of individuals presenting acute viral syndrome with unknown etiology indicates that a rich yield of new human viruses may be readily identifiable using simple methods of sequence-independent nucleic acid amplification and limited sequencing.
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页码:8230 / 8236
页数:7
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