Bioinspired polysaccharide hybrid hydrogel promoted recruitment and chondrogenic differentiation of bone marrow mesenchymal stem cells

被引：55

作者：

Li, Zhulian ^{[1
,2
]}

Cao, Hongfu ^{[1
,2
]}

Xu, Yang ^{[1
,2
]}

Li, Xing ^{[1
,2
]}

Han, XiaoWen ^{[1
,2
]}

Fan, Yujiang ^{[1
,2
]}

Jiang, Qing ^{[3
]}

Sun, Yong ^{[1
,2
]}

Zhang, Xingdong ^{[1
,2
]}

机构：

[1] Sichuan Univ, Natl Engn Res Ctr Biomat, 29 Wangjiang Rd, Chengdu 610064, Sichuan, Peoples R China

[2] Sichuan Univ, Coll Biomed Engn, 29 Wangjiang Rd, Chengdu 610064, Peoples R China

[3] Sichuan Univ, Coll Mat Sci & Engn, 29 Wangjiang Rd, Chengdu 610064, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2021年 / 267卷

关键词：

Polysaccharide hybrid hydrogel; Biomimetic cartilage matrix; rBMSCs; Recruitment; Chondrogenic differentiation; HYALURONIC-ACID HYDROGEL; CARTILAGE REPAIR; IN-VITRO; MICROFRACTURE; SCAFFOLDS; COLLAGEN; MATRIX; KNEE;

D O I：

10.1016/j.carbpol.2021.118224

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Cartilage regeneration by biomimetic cartilage matrix with synchronously recruited stem cells was one of ideal strategies. Inspired by catechol for proteins adhesion, dopamine modified polysaccharide hybrid hydrogel (HDC) was prepared by integrating collagen I (Col I) and hyaluronic acid derivatives (HA-DN) with sulfhydryl modified polysaccharide hybrid hydrogel (HS-C) as control. Because of double-crosslinking architecture, HD-C hydrogel was endowed with a more compact pore structure, higher mechanical properties and water retention ability in comparison with those of HS-C hydrogel. Meanwhile, it significantly promoted the proliferation and spread of rabbit bone marrow stem cells (rBMSCs), and accelerated cartilaginous matrix secretion. RT-PCR results also verified higher related gene expression of chondrogenesis (Sox 9, Agg and Col II). Moreover, HD-C hydrogel could enhance the enrichment and migration of rBMSCs in vitro by potential functional protein adsorption mechanisms, and this phenomenon was further confirmed by more rBMSCs migration in short-term joint implantation experiments in vivo.

引用

页数：13

共 44 条

[1]

Engineering of gradient osteochondral tissue: From nature to lab [J].