Chromosome variability of human multipotent mesenchymal stromal cells

被引：33

作者：

Bochkov, N. P.

Voronina, E. S. ^{[1
]}

Kosyakova, N. V.

Liehr, T.

Rzhaninova, A. A.

Katosova, L. A.

Platonova, V. I.

Gol'dshtein, D. V.

机构：

[1] IM Setchenov Moscow Med Acad, Moscow, Russia

[2] Russian Acad Med Sci, Med Genet Res Ctr, Moscow, Russia

[3] ReMeTeks Co, Moscow, Russia

[4] Univ Jena, Inst Human Genet & Anthropol, D-6900 Jena, Germany

来源：

BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2007年 / 143卷 / 01期

基金：

俄罗斯基础研究基金会;

关键词：

multipotent mesenchymal stromal cells; karyotyping; aneuploidy; chromosome aberrations;

D O I：

10.1007/s10517-007-0031-0

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

We elaborated a method of preparing cytogenetic preparations of cultured multipotent mesenchymal stromal cells from the adipose tissue. It was found that karyotypic changes (monosomy, translocations) appear in some samples during culturing. Clones with changed karyotype were detected in 11-14-passage cultures from 2 of 7 individuals. The percent of aberrant cells in cultures from different individuals varied from 1.5 to 5.95 per 100 cells, which attested to karyotype instability. These data substantiate the need for cytogenetic control of cells before their transplantation into donor organism and further investigation of chromosome variability in stem cells.

引用

收藏

页码：122 / 126

页数：5

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