Bioinformatic and expression analysis of the putative gliotoxin biosynthetic gene cluster of Aspergillus fumigatus

被引：189

作者：

Gardiner, DM ^{[1
]}

Howlett, BJ ^{[1
]}

机构：

[1] Univ Melbourne, Sch Bot, Melbourne, Vic 3010, Australia

来源：

FEMS MICROBIOLOGY LETTERS | 2005年 / 248卷 / 02期

基金：

英国惠康基金;

关键词：

epipolythiodioxopiperazine; sirodesmin; Aspergillosis; peptide synthetase; qRT-PCR; RIP mutation;

D O I：

10.1016/j.femsle.2005.05.046

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Gliotoxin is a secondary metabolite produced by several fungi including the opportunistic animal pathogen Aspergilli is fumigatus tits. It is a member of the epipolythiodioxopiperazine (ETP) class of toxins characterised by a disulphide bridged cyclic dipeptide. A putative cluster of 12 genes involved in gliotoxin biosynthesis has been identified in A. fumigatus by a comparative genomics approach based on homology to genes from the sirodesmin (another ETP) biosynthetic gene cluster of Leptosphaeria maculans. The physical limits of the cluster in A. fumigatus have been defined by bioinformatics and by identifying the genes that are co-regulated and whose timing of expression correlates with the production of gliotoxin in culture. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：241 / 248

页数：8

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