Glutamine synthetases of Corynebacterium glutamicum:: transcriptional control and regulation of activity

被引：60

作者：

Nolden, L

Farwick, M

Krämer, R

Burkovski, A

机构：

[1] Univ Cologne, Inst Biochem, D-50674 Cologne, Germany

[2] Degussa AG, D-33788 Halle, Germany

来源：

FEMS MICROBIOLOGY LETTERS | 2001年 / 201卷 / 01期

关键词：

adenylyltransferase; ammonium assimilation; glutamine synthetase; nitrogen control; Corynebacterium glutamicum;

D O I：

10.1016/S0378-1097(01)00244-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Regulation of glnA expression and glutamine synthetase I activity was analyzed in Corynebacterium glutamicum. Transcription is regulated by the global repressor protein AmtR, essential for derepression ol glnA transcription are GlnK and uridylyltransferase, key proteins of the C. glutamicum nitrogen regulatory system. Glutamine synthetase I activity is controlled by adenylylation/deadenylylation via adenylyltransferase. The gene encoding this bifunctional enzyme, glnE, was isolated and its function was characterized by deletion analysis. Upstream of glnE, a second gene encoding a GSI-type protein in C. glutamicum was isolated. This gent, designated gln, 12, forms an operon with glnE, its transcription is not regulated and neither its deletion or overexpression showed any effect. Therefore. the physiological role of glnA2 remains unclear. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：91 / 98

页数：8

共 23 条

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