Cloning and expression and immunogenicity of Helicobacter pylori BabA2 gene

被引：9

作者：

Bai, Yang ^{[1
]}

Zhang, Ya-Li ^{[1
]}

Chen, Ye ^{[1
]}

Jin, Jian-Feng ^{[2
]}

Zhang, Zhao-Shan ^{[3
]}

Zhou, Dian-Yuan ^{[1
]}

机构：

[1] First Mil Med Univ, Nanfang Hosp, PLA Inst Digest Med, Guangzhou 510515, Guangdong, Peoples R China

[2] Chem Univ Beijing, Beijing 100071, Peoples R China

[3] Acad Mil Med Sci, Inst Biotechnol, Beijing 100071, Peoples R China

来源：

WORLD JOURNAL OF GASTROENTEROLOGY | 2004年 / 10卷 / 17期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.3748/wjg.v10.i17.2560

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

AIM: To construct a recombinant strain which expresses BabA of Helicobacter pylori (H pylori) and to study the immunogenicity of BabA. METHODS: BabA(2) DNA was amplified by PCR and inserted into the prokaryotie expression vector pET-22b (+) and expressed in the BL21 (DE3) E. coli strain. Furthermore, BabA immunogenicity was studied by animal test. RESULTS: DNA sequence analysis showed the sequence of BabA(2) DNA was the same as the one published by GenBank. The BabA recombinant protein accounted for 34.8% of the total bacterial protein. The serum from H pylori infected patients and Balb/c miced immunized with BabA itself could recognize rBabA. CONCLUSION: BabA recombinant protein may be an potential vaccine for control and treatment of H pylori infection.

引用

页码：2560 / 2562

页数：3

共 11 条

[1]

Bai Yang, 2002, Di Yi Jun Yi Da Xue Xue Bao, V22, P869

[2]

Bai Yang, 2003, Di Yi Jun Yi Da Xue Xue Bao, V23, P101

[3]

Bai Yang, 2003, Di Yi Jun Yi Da Xue Xue Bao, V23, P293

[4] Salivary MUC5B-mediated adherence (ex vivo) of Helicobacter pylori during acute stress [J].

Bosch, JA ;

de Geus, EJC ;

Ligtenberg, TJM ;

Nazmi, K ;

Veerman, ECI ;

Hoogstraten, J ;

Amerongen, AVN .

PSYCHOSOMATIC MEDICINE, 2000, 62 (01) :40-49

[5] MOLECULAR CHARACTERIZATION OF THE 128-KDA IMMUNODOMINANT ANTIGEN OF HELICOBACTER-PYLORI-ASSOCIATED WITH CYTOTOXICITY AND DUODENAL-ULCER [J].

COVACCI, A ;

CENSINI, S ;

BUGNOLI, M ;

PETRACCA, R ;

BURRONI, D ;

MACCHIA, G ;

MASSONE, A ;

PAPINI, E ;

XIANG, ZY ;

FIGURA, N ;

RAPPUOLI, R .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (12) :5791-5795

[6] Helicobacter pylori adhesin binding fucosylated histo-blood group antigens revealed by retagging [J].

Ilver, D ;

Arnqvist, A ;

Ögren, J ;

Frick, IM ;

Kersulyte, D ;

Incecik, ET ;

Berg, DE ;

Covacci, A ;

Engstrand, L ;

Borén, T .

SCIENCE, 1998, 279 (5349) :373-377

[7]

Morgner A, 2001, WORLD J GASTROENTERO, V7, P248

[8]

Sambrook J, 1989, MOL CLONING LAB MANU

[9] Immunization with recombinant Helicobacter pylori urease in specific-pathogen-free rhesus monkeys (Macaca mulatta) [J].

Solnick, JV ;

Canfield, DR ;

Hansen, LM ;

Torabian, SZ .

INFECTION AND IMMUNITY, 2000, 68 (05) :2560-2565

[10] Helicobacter pylori infection [J].

Vandenplas, Y .

WORLD JOURNAL OF GASTROENTEROLOGY, 2000, 6 (01) :20-31

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