Proposed pathway for the biosynthesis of serovar-specific glycopeptidolipids in Mycobacterium avium serovar 2

被引:38
作者
Eckstein, TM [1 ]
Belisle, JT [1 ]
Inamine, JM [1 ]
机构
[1] Colorado State Univ, Dept Microbiol Immunol & Pathol, Mycobacteria Res Labs, Ft Collins, CO 80523 USA
来源
MICROBIOLOGY-SGM | 2003年 / 149卷
关键词
D O I
10.1099/mic.0.26528-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Members of the Mycobacterium avium complex are distinguished by the presence of highly antigenic surface molecules called glycopeptidolipids (GPLs) and the oligosaccharide portion of the serovar-specific GPL defines the 28 serovars. Previously, the genomic region (ser2) encoding the enzymes responsible for the glycosylation of the lipopeptide core to generate the serovar-2-specific GPLs has been described. In this work, the ser2 gene clusters of M. avium serovar 2 strains 2151 and TMC 724 were fully sequenced and compared to the homologous regions of M. avium serovar 1 strain 104, M. avium subsp. paratuberculosis and M. avium subsp. silvaticum. It was also determined that 104Rg, a mutant of strain 104 that produces truncated GPLs, lost several GPL biosynthesis genes by deletion. This comparison, together with analysis of protein similarities, supports a biosynthetic model in which serovar-2-specific GPLs are synthesized from a serovar-1-specific GPL intermediate that is derived from a non-specific GPL precursor. We also identified a gene encoding an enzyme that is necessary for the biosynthesis of serovar-3- and 9-specific GPLs, but not serovar-2-specific GPLs, suggesting that the different serovars may have evolved from the acquisition or loss of genetic information. In addition, a subcluster of genes for the biosynthesis and transfer of fucose, which are needed to make serovar-specific GPLs such as those of serovar 2, is found in the non-GPL-producing M. avium subspecies para tuberculosis and silvaticum.
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页码:2797 / 2807
页数:11
相关论文
共 39 条
[1]   Identification of the fucose synthetase gene in the colanic acid gene cluster of Escherichia coli K-12 [J].
Andrianopoulos, K ;
Wang, L ;
Reeves, PR .
JOURNAL OF BACTERIOLOGY, 1998, 180 (04) :998-1001
[2]   GENOMIC ORGANIZATION OF THE KLEBSIELLA-PNEUMONIAE CPS REGION RESPONSIBLE FOR SEROTYPE K2 CAPSULAR POLYSACCHARIDE SYNTHESIS IN THE VIRULENT-STRAIN CHEDID [J].
ARAKAWA, Y ;
WACHAROTAYANKUN, R ;
NAGATSUKA, T ;
ITO, H ;
KATO, N ;
OHTA, M .
JOURNAL OF BACTERIOLOGY, 1995, 177 (07) :1788-1796
[3]   The variable surface glycolipids of mycobacteria: Structures, synthesis of epitopes, and biological properties [J].
Aspinall, GO ;
Chatterjee, D ;
Brennan, PJ .
ADVANCES IN CARBOHYDRATE CHEMISTRY AND BIOCHEMISTRY, VOL 51, 1995, 51 :169-242
[4]   ISOLATION AND EXPRESSION OF A GENE-CLUSTER RESPONSIBLE FOR BIOSYNTHESIS OF THE GLYCOPEPTIDOLIPID ANTIGENS OF MYCOBACTERIUM-AVIUM [J].
BELISLE, JT ;
PASCOPELLA, L ;
INAMINE, JM ;
BRENNAN, PJ ;
JACOBS, WR .
JOURNAL OF BACTERIOLOGY, 1991, 173 (21) :6991-6997
[5]  
BELISLE JT, 1993, J BIOL CHEM, V268, P10517
[6]  
BELISLE JT, 1993, J BIOL CHEM, V268, P10510
[7]  
Brennan P., 1988, MICROBIAL LIPIDS, V1, P203
[8]  
BRENNAN PJ, 1979, J BIOL CHEM, V254, P4205
[9]   Further studies on the GS element -: A novel mycobacterial insertion sequence (IS1612), inserted into an acetylase gene (mpa) in Mycobacterium avium subsp silvaticum but not in Mycobacterium avium subsp paratuberculosis [J].
Bull, TJ ;
Sheridan, JM ;
Martin, H ;
Sumar, N ;
Tizard, M ;
Hermon-Taylor, J .
VETERINARY MICROBIOLOGY, 2000, 77 (3-4) :453-463
[10]   The surface glycopeptidolipids of mycobacteria: structures and biological of properties [J].
Chatterjee, D ;
Khoo, KH .
CELLULAR AND MOLECULAR LIFE SCIENCES, 2001, 58 (14) :2018-2042