Proposed pathway for the biosynthesis of serovar-specific glycopeptidolipids in Mycobacterium avium serovar 2

被引:38
作者
Eckstein, TM [1 ]
Belisle, JT [1 ]
Inamine, JM [1 ]
机构
[1] Colorado State Univ, Dept Microbiol Immunol & Pathol, Mycobacteria Res Labs, Ft Collins, CO 80523 USA
来源
MICROBIOLOGY-SGM | 2003年 / 149卷
关键词
D O I
10.1099/mic.0.26528-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Members of the Mycobacterium avium complex are distinguished by the presence of highly antigenic surface molecules called glycopeptidolipids (GPLs) and the oligosaccharide portion of the serovar-specific GPL defines the 28 serovars. Previously, the genomic region (ser2) encoding the enzymes responsible for the glycosylation of the lipopeptide core to generate the serovar-2-specific GPLs has been described. In this work, the ser2 gene clusters of M. avium serovar 2 strains 2151 and TMC 724 were fully sequenced and compared to the homologous regions of M. avium serovar 1 strain 104, M. avium subsp. paratuberculosis and M. avium subsp. silvaticum. It was also determined that 104Rg, a mutant of strain 104 that produces truncated GPLs, lost several GPL biosynthesis genes by deletion. This comparison, together with analysis of protein similarities, supports a biosynthetic model in which serovar-2-specific GPLs are synthesized from a serovar-1-specific GPL intermediate that is derived from a non-specific GPL precursor. We also identified a gene encoding an enzyme that is necessary for the biosynthesis of serovar-3- and 9-specific GPLs, but not serovar-2-specific GPLs, suggesting that the different serovars may have evolved from the acquisition or loss of genetic information. In addition, a subcluster of genes for the biosynthesis and transfer of fucose, which are needed to make serovar-specific GPLs such as those of serovar 2, is found in the non-GPL-producing M. avium subspecies para tuberculosis and silvaticum.
引用
收藏
页码:2797 / 2807
页数:11
相关论文
共 39 条
[21]   Plant O-methyltransferases:: molecular analysis, common signature and classification [J].
Ibrahim, RK ;
Bruneau, A ;
Bantignies, B .
PLANT MOLECULAR BIOLOGY, 1998, 36 (01) :1-10
[22]  
INOUYE M, 1994, GENE, V141, P121
[23]   FramePlot: a new implementation of the Frame analysis for predicting protein-coding regions in bacterial DNA with a high G plus C content [J].
Ishikawa, J ;
Hotta, K .
FEMS MICROBIOLOGY LETTERS, 1999, 174 (02) :251-253
[24]   Modification of glycopeptidolipids by an O-methyltransferase of Mycobacterium smegmatis [J].
Jeevarajah, D ;
Patterson, JH ;
McConville, MJ ;
Billman-Jacobe, H .
MICROBIOLOGY-SGM, 2002, 148 :3079-3087
[25]   THE GENETICS OF ENCAPSULATION IN HAEMOPHILUS-INFLUENZAE [J].
KROLL, JS .
JOURNAL OF INFECTIOUS DISEASES, 1992, 165 :S93-S96
[26]   Characterization of genetic differences between Mycobacterium avium subsp avium strains of diverse virulence with a focus on the glycopeptidolipid biosynthesis cluster [J].
Krzywinska, E ;
Schorey, JS .
VETERINARY MICROBIOLOGY, 2003, 91 (2-3) :249-264
[27]   Molecular analysis of genetic differences between Mycobacterium bovis BCG and virulent M-bovis [J].
Mahairas, GG ;
Sabo, PJ ;
Hickey, MJ ;
Singh, DC ;
Stover, CK .
JOURNAL OF BACTERIOLOGY, 1996, 178 (05) :1274-1282
[28]   Hemolysin as a virulence factor for systemic infection with isolates of Mycobacterium avium complex [J].
Maslow, JN ;
Dawson, D ;
Carlin, EA ;
Holland, SM .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (02) :445-446
[29]   LOCI OF MYCOBACTERIUM-AVIUM SER2 GENE-CLUSTER AND THEIR FUNCTIONS [J].
MILLS, JA ;
MCNEIL, MR ;
BELISLE, JT ;
JACOBS, WR ;
BRENNAN, PJ .
JOURNAL OF BACTERIOLOGY, 1994, 176 (16) :4803-4808
[30]   Identification of a methyltransferase from Mycobacterium smegmatis involved in glycopeptidolipid synthesis [J].
Patterson, JH ;
McConville, MJ ;
Haites, RE ;
Coppel, RL ;
Billman-Jacobe, H .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (32) :24900-24906